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原发性肝内胆管结石的细菌学和电镜观察研究 被引量:11

Bacteriological and electron microscopic observation of primary intrahepatic cholelithiasis
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摘要 目的探讨原发性肝内胆管结石与胆道感染的关系。方法手术过程中,在无菌条件下收集35例原发性肝内胆管结石病人的胆管胆汁35份,结石30份,进行需氧菌培养,并对部分结石行扫描电镜和透射电镜观察。结果胆汁细菌培养阳性率为94.2%,结石细菌培养阳性率为96.7%。胆汁和胆石培养出的细菌种类相似,均以肠球菌属细菌占首位,其次为大肠杆菌和克雷伯菌属。对6例肝内胆管结石行扫描电镜观察,5例行透射电镜观察,均观察到结石内有细菌存在,这11例病人的胆汁、胆石细菌培养均呈阳性。结论原发性肝内胆管结石内有细菌存在,可能是引起胆道感染的重要感染源之一。 Objective To discuss the relationship between the primary intrahepatic biliary stones and biliary infection.Methods Thirty-five bile specimens and 30 stone specimens were collect- ed from patients with intrahepatic cholelithiasis during the surgery.They were analyzed by bacteria culture and part of the stone specimens were examined by SEM and TEM.Results The positive rate of culture was 94.2% and 96.7% for the bile specimens and stone ones,respectively.Similar bacteria were found in the bile and stone.Enteral coccus was the most common one,which followed in order by E.coli and Klebesilla.Respective examinations of SEM and TEM in 6 stone specimens and the oth- er 5 showed that bacteria existed in all the specimens.Conclusions Bacteria exist in the primary intra- hepatic biliary stones,which might be one of the causes for biliary infection.
出处 《中华肝胆外科杂志》 CAS CSCD 2001年第12期717-720,共4页 Chinese Journal of Hepatobiliary Surgery
关键词 细菌学 电镜观察 原发性肝内胆管结石 胆道感染 Cholelithiasis Bacteria Electronic microscopy
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