摘要
两株人胃癌细胞,MKN-28和MKN-45,分皮下、腹腔、胃壁和尾静脉途径种植在无胸腺裸鼠体内。腹腔和胃壁种植后荷瘤鼠存活时间较短,肿瘤相关死亡率高。而经皮下种植后荷瘤鼠存活最长。尾静脉接种后易在肺和脑形成转移瘤。种植在腹腔和胃壁的癌细胞能自发性转移到肝、食管旁组织、纵膈和肺。而种植在皮下的癌细胞不形成转移灶。肠系膜、肝、胰、结肠、胃和膈肌易受癌细胞侵袭,小肠和脾均不受累及。证明不同种植部位的组织和器官特性同癌细胞在体内的生物学行为有密切关系。
In this study, two human gastric cancer cell lines, MKN-28 and MKN-45, were employed. The tumor cells were inoculated by subcutaneous(s, c.), intraperitoneal (i. p.), gastric wall (g. w.) and intravenous (i. v.) routes into athymic nude mice. Although both tumor cell lines were tumorogenic after all four routes of inculation, their propagation efficiency, invasive abilities and metastasis varied with the routes of inoculation. The survival time and tumor-related mortality of tumor-bearing mice were closely related to the sites of inocul ation. The shortest survival among all tumor-bearing mice was in those inoculated by i.p. route, followed by g. w. route, i. v. route, and the longest survival was in the mice inoculated by s. c. route. The tumor cells have a preference for growing on some organs, including the mesentery, liver, pancreas, colon, stomach as well as the diaphragm, while sparing the small intestine and the spleen. After i. v. inoculation, all mice developed experimental pulmonary metastasis. Some of them also formed extrapulmonary metastasis simultaneously, most commonly in the brain. Besides, the tumor cells also showed an ability of spontaneous metastasis to some organs and tissues, including the liver, mediastinum, lung and periesophageal tissues after i.p. and g.w. inoculations. These date demonstrate that the biological behavior of cancer cells is influenced by the routes of inoculation, and organ-site specific inoculation of tumor cells is essential for optimal growth and progression of tumors in vivo.
出处
《上海第二医科大学学报》
CSCD
1991年第3期193-198,共6页
Acta Universitatis Medicinalis Secondae Shanghai
基金
国家自然科学基金
关键词
胃癌
转移
癌细胞
动物模型
gastric cancer tumor metastasis athymie nude mice animal mode
