摘要
采用mRNA差异显示技术对不同催青处理的伴性赤蚁sch系统进行研究 ,并以普通黑蚁系统夏芳作为对照。实验中分别抽提高温和常温催青处理 36h后的sch蚕卵和夏芳蚕卵的总RNA进行DDRT -PCR分析 ,其产物于变性聚丙烯酰胺凝胶上电泳展示 ,得到大量的差示片段。对高温催青处理后在夏sch蚕卵中特异表达的 2条能稳定重复的带进行了克隆 ,得到了长度分别为 2 73和 199bp的cDNA片段 ,并对其进行了序列分析。Northern杂交证实HAP82 80仅在高温催青处理的夏sch蚕卵中有转录 。
In this study,the total RNAs of sch eggs which had been incubated for 36h under high temperature and normal temperature respectively were extracted to analyze by DDRT PCR.The products of PCR were resolved by electrophoresis in 6% denaturing polyacrylamide gel,two specific fragmets from Xia sch /high which could complicate steadily were cut out and cloned.One is 273 bp,the other is 199 bp,both cDNA fragments were sequenced and their homology were analyzed.Northern blotting proved HAP 8280 was transcripted only in the Xia sch /high,which revealed HAP 8280 was real expression product of Xia sch /high.
出处
《蚕业科学》
CAS
CSCD
2001年第4期267-271,共5页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金资助项目 (编号 :30 0 70 5 80 )。
