摘要
为探索一种能准确、快速测定三唑磷和仲丁威并存时的方法 ,采用反相 C1 8柱对三·仲乳油中三唑磷和仲丁威的高效液相色谱测定方法进行了研究 .结果表明 ,以 V(乙腈 )∶ V(水 ) =5 5∶ 45为流动相 ,在波长 2 6 2 nm下检测 ,三唑磷和仲丁威的变异系数分别为 0 .6 9%和 0 .83%,平均回收率分别为 10 0 .0 7%和 99.76 %.三唑磷和仲丁威的质量浓度分别在 0 .0 15～ 1.2 70 m g/ m L 和 0 .0 0 6～ 2 .5 40 m g/ m L 的范围内线性关系良好 .
A reversed-phase high performance liquid chromatographic method for the separation and determination of triazophos and fenobucarb in the composite emulsion is developed.The sample was separated on Hypersil BDS C 18 column 250 mm×4 mm i.d ,acetonitrile ∶ water (55 ∶ 45,v/v) as mobile phase. It was then detected at 262 nm by DAD detector. Quantification was carried out with the two external standards .The average recovery was 100.07% with a coefficient of variation of 0.69% for triazophos and 99.76% with a coefficient of variation of 0.83% for fenobucarb .The linear range of this method was 0.015～1.270 mg/mL(r=0.999 9) for triazophos and 0.006～ 2.540 mg/mL (r=0.999 9) for fenobucarb.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第6期486-487,共2页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南农业大学青年科研基金项目 (2 0 0 1- 0 12 )
