摘要
应用‘Myo’小卫星 DNA 探针,Southern 印迹杂交技术,对血斑、精斑、同一个体不同组织进行 DNA 指纹图分析,均获得清晰的图谱。同一个体的血斑与血液、精斑与精液以及不同的组织其 DNA 指纹图谱完全相同。可以根据斑痕或组织与嫌疑个体的血液或某一组织 DNA 的指纹图谱比对以做出同一认定。50μl 血液量的血斑、5μl 精液量的精斑可以获得清晰易辨的指纹图谱。五年的精斑、两年的血斑亦可做出与同源个体新鲜精液、血液完全一致的 DNA 指纹图谱。对杀人、强奸杀人、碎尸等不同案件的血痕、精斑、不同组织碎块进行了 DNA 指纹图检验,均做出了正确的个体认定。本方法的应用为我国法医物证检验提供了新的分析手段,使个体认定得以实现。
DNA fingerprints analysis of Chinese population were conducted by Myo mini-satellite DNA probes with elbow venous blood samples collected from 16 personsof two family and 100 unrelated individuals,Distinct DNA fingerprints were ob-tained when the DNA extracted from blood samples were digested with the Hinf Ⅰor Hae Ⅱ,and Southern blot hybridized with Myo minisatellite probe.Theresulting showed that more than ten hybridized bands could be detected of eachindividual.The chance of band sharing among-the individuals is<4×10^(-9),so thefingerprint patterns obtained Were individual specific.Hybridized bands wereinherited according to the Mendelian dominant fashion.In two paternity cases,some of the hybridized bands could be detected in the maternal side,the rest ofthem could be found in the suspected father's,thus confirmed the suspected fatheras biological father.MYO DNA probes have a great practical value in the paternitytest as well as in the personnal identification.
出处
《中国法医学杂志》
CSCD
1990年第2期65-68,共4页
Chinese Journal of Forensic Medicine
关键词
血斑
DNA指纹图
法医学鉴定
精斑
DNA fingerprints DNA polymorphism Personnal identification Paterniy test Probe
