摘要
目的 观察诱导分化剂苯乙酸对C6胶质瘤细胞增殖分化过程中抑癌基因p16的蛋白水平表达变化的影响。方法 3 H -TdR掺入法测细胞增殖率 ;免疫组化SP法检测p16基因蛋白水平的表达。结果 应用苯乙酸后 ,细胞cpm值降低 ;p16基因胞浆阳性表达显著升高。结论 苯乙酸对胶质瘤细胞存在剂量依赖性抑制 ,增强了抑癌基因p16表达活性 。
Abstrait:Objective To determine the growth-inhibitory and differentiation-induing activity of phenylacetate (PA)on glioma cell line C6,to find the change of p16 gene expression.Methods The effect of PA on cell proliferation using 3 H-TdR assay, P16 gene protein expression studied by immunohistochemistry technique.Results At does ranges 0.625?2 5?5 0 nmol/L,PA caused a dose dependent proliferation inhibitory effect. The protein expression of pl6 gene was notably increased by PA.Conclusion PA caused a does-edpendent proliferation inhibitory effect,the expression of p16 gene activity increased by PA.
出处
《中国实验诊断学》
2002年第1期16-17,共2页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金资助项目 ( 3990 0 15 2 )
