摘要
目的 研究正常肝细胞和肝癌细胞流变特性与细胞骨架结构的相关性。方法 以微管吸吮技术测定两种细胞的粘弹性系数和细胞在２μｇ／ｍｌ Ⅳ型胶原则１．２５ μｇ／ｍｌ层粘连蛋白（ｌａｍｉｎｉｎ， ＬＮ）复台裱衬表面上的粘附力，以秋水仙素（ｃｏｌｃｈｉｃｉｎｅ，Ｃｏｌ）和细胞松弛素Ｄ（ｃｙｔｏｃｈｌａｓｉｎ，ＣＤ）处理细胞研究其对细胞粘弹性系数和粘附力的影响。结果 在质量浓度为１～６０ ｍｇ／Ｌ的Ｃｏｌ作用下，肝细胞弹性系数及粘附力总体上明显增高或趋于增高，而肝癌细胞粘弹性系数及粘附力明显降低；在质量浓度为０．２５～ ５００ ｍｇ／Ｌ的ＣＤ作用下，两种细胞粘弹性系数及粘附力均明显降低，肝癌细胞弹性系数Ｋ１、Ｋ２值及粘附力的下降幅度明显大于肝细胞相应值的下降幅度。细胞在Ⅳ型胶原／ＬＮ复合裱衬表面的粘附力与细胞粘弹性系数呈相同的变化趋势，肝癌细胞粘附力变化与细胞弹性系数Ｋ１、 Ｋ２的变化呈高度正相关（Ｐ＜０．０１）。结论 在ＣＤ和Ｃｏｌ作用下肝细胞和肝癌细胞的流变特性呈不同的效应方式或强度，反映了肝细胞和肝癌细胞细胞骨架结构和机能状态的差异。
Objective To investigate the relevance of the rheological properties, i.e., viscoelasticities and adhesion to basement membrane components coated surface, of both hepatocytes and hepatocellular carcinoma (HCC) cells to the cytoskeleton structure. Methods Micropipette aspiration technique was adopted to measure viscoeiastic coefficients and adhesion forces to 2μg/ml collagen Ⅳ/1 .25 μg/ml laminin coated surface of the cells. Two kinds of cytoskeleton perturbing agents, colchicine and cytochalasin D, were used to treat both HCC cells and hepatocytes and the effects of these treatments on cell viscoelastic coefficients and cell adhesion forces were investigated. Results Upon treatment of cells with colchicine in a concentration range of 1-60 mg/L, the elastic coefficients, especially the first elastic coefficient K1, and adhesion forces of hepatocytes generally tended to increase or increased significantly while, in contrast, viscicoelastic coefficients and adhesion forces of HCC cells decreased obviously. Upon treatment of cells with cytochalasin D in a concentration range of 0.255.00 mg/L, viscoelastic coefficients of both hepatocytes and HCC cells decreased uniformly, with a larger magnitude for the decrease in elastic coefficients and adhesion forces of HCC cells than for those of hepatocytes. Adhesion forces of hepatocytes and HCC cells onto collagen Ⅳ/laminin coated surface varied similarly as viscoelastic coefficients under the action of the cytoskeleton perturbing agents. A significant positive correlation existed between changes of HCC cell adhesion forces on collagen Ⅳ/laminin coated surfaces and those of cell elastic coefficients(P< 0.01). Conclusions The effects of colchicine and cytochalasin D on rheological properties of HCC cells differ significantly either in ways or extents from those on rheological properties of hepatocytes. These results might reflect the difference in the state of cytoskeleton structure and function among these two kinds of cells.
出处
《中华肝脏病杂志》
CAS
CSCD
2001年第1期25-27,共3页
Chinese Journal of Hepatology
基金
国家自然科学基金!(39370198
39500037
19732003-1)
关键词
肝癌
细胞
细胞粘附
细胞骨架
细胞流变学
Carcinoma, hepatocellular
Cell adhesion
Cytoskeleton
Cell rheology
