摘要
目的 探讨蛙皮素对新生兔胃肠形态学及胃肠粘膜细胞增殖的调节作用。 方法 新生兔 2 4只 ,随机分为蛙皮素大、小剂量 [15μg/ (kg· d)及 30μg/ (kg· d) ]实验组及对照组 ,每组 8只 ,应用图像分析技术及电子显微镜检测新生兔胃肠形态学变化 ,并应用免疫组织化学技术测定胃肠粘膜上皮细胞增殖率。 结果 (1)新生兔大、小剂量组十二指肠绒毛高度分别为 (5 2 0± 76 )、(5 13±31)μm,较对照组的 (379± 44 )μm显著增高 (P <0 .0 0 1) ;空肠绒毛高分别为 (5 12± 37)、(4 2 7± 5 4)μm ,较对照组的 (372± 2 9)μm显著增高 (P<0 .0 5 ) ;回肠绒毛高度分别为 (4 6 8± 138)、(5 2 0± 112 )μm ,较对照组的 (2 5 3± 6 0 )μm显著增高 (P<0 .0 5 )。 (2 )蛙皮素能够使肠粘膜上皮细胞更为成熟 ,且胃肠粘膜细胞增殖率亦显著增加 (P<0 .0 5 )。 结论 蛙皮素在一定程度上能够促进胃肠形态发育 。
Objective To investigate the regulatory effects of bombesin on the gastrointestinal morphology and proliferation of mucosa cells in neonatal rabbits. Methods Twenty four neonatal rabbits were divided into big,small dose experimental group and control group. The gastrointestinal morphology in neonatal rabbits was observed by using Video Image Digtal Analysis System and electron microscopy, and the proliferative rate of gastrointestinal epithelium cells was detected by using immunohistochemical assay. Results The villous height of duodenum were (520±76),(513±31),(379±44) μm in three groups respectively. That in experimental group with big or small dose were significantly higher than that in control group( P< 0.001). The villous height of jejunum were (512±37),(427±54),(372±29) μm in three groups respectively. The villous height of jejunum in experimental group with big or small dose were significantly higher than that in control group( P< 0.05). The villous height of ileum were (468±138),(520±112),(253±60) μm in three groups respectively. That in experimental group with big or small dose were significantly higher than that in control group( P< 0.05). Moreover, bombesin could stimulate intestinal epithelium cells maturation and increase the proliferative rate of mucosa cells in neonatal gut( P< 0.05). Conclusion Bombesin could affect the morphology of gut at certain degree and stimulate proliferation of mucosa cells in neonatal rabbits.
出处
《中华围产医学杂志》
CAS
2001年第4期243-246,共4页
Chinese Journal of Perinatal Medicine
