摘要
在ｐＨ３．４～４．０的缓冲溶液中，偶氮胂Ｍ（ＡＡＭ）、偶氮氯膦Ⅲ（ＣＰＡⅢ ）和氯磺酚Ｓ（ＣＳＰＳ）等变色酸双偶氮染料及蛋白质本身的共振瑞利散射（ＲＲＳ）均十分微弱，但这些染料与蛋白质结合形成复合物时能使ＲＲＳ急剧增强，在４００～４７０ｎｍ的范围内呈现高的散射强度。其最大散射波长均位于４７０ｎｍ处。并且散射强度分别在０～３．６ｍｇ／Ｌ（ＣＰＡⅢ体系）、０～３、８ｍｇ／Ｌ（ＡＡＭ体系）和０～４．８ｍｇ／Ｌ（ＣＳＰＳ体系）的范围内与牛血清白蛋白（ＢＳＡ）的浓度成正比，方法具有高灵敏度，对于 ＢＳＡ的检出限（σ＝３时）分别为１８．５ μｇ／Ｌ（ＣＰＡ Ⅲ）、１３．６ μｇ／Ｌ（ＣＳＰ Ｓ）和 ２７．９ μｇ／Ｌ（ＡＡ Ｍ）。考察了共存物质的影响，表明方法具有较好的选择性。此法可用于人血清中蛋白质的测定。
In a medium of citric acid-HCl buffer (pH 3.4similar to4.0), some bisazo dyes of chromatropic acid such as arsenazo M (AA M), chlorophosphonazo III (CPA III) and chlorosulphonphenol S (CSP S) combine with proteins, causing enhanced resonance Rayleigh scattering (RRS) in the range of 400similar to470 nm and the maximum RRS peak at 470 nm. Their calibration graphs are linear over the range of 0similar to3.6 mg/L for CPA III-bovine serum albumin (BSA) system, 0similar to3.8 mg/L for A-A M-BSA system and 0similar to4.8 mg/L for CSP S-BSA system separately. The method has high sensitivity with the detection limits of BSA (sigma = 3) 18.5mug/L (CPA III), 13.6 mug/L (CSP S) and 27.9 mug/L (A-A M). This method can be applied to the determination of total protein in human serum.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2002年第1期81-85,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助项目(No.29875019)
