摘要
以所筛选的硅酸盐细菌RM 0 1菌株为材料 ,对所需的培养基和培养条件进行了研究分析 .在选择的适宜培养基中 (TR复合培养基 ) ,对培养基pH值、培养温度、摇床转速及摇瓶装液量等影响因素进行了正交实验 .结果表明 ,在pH为 7、温度 36℃、转速 2 2 0r/min、装液量 5 0mL时 ;硅酸盐细菌菌量可达 10 .6× 10 8个
This paper dealt with Bacillus mucilaginosus cultured and condition optimized,culture experiment was carried on using a serial of media.The results indicated that medium composition,pH,inoculation volume effected the growth of Bacillus mucilaginosus.A kinds of low-price and availabilty culture medium was developed for the culture of Bacillus mucilaginosus.The 10.6×10 8 bacteria cells/mL was obtained in experiment conditon by design orthogonal experiment.The optimizing condition is pH7,36℃,220?r/min,and 50?mL medium volume.
出处
《南京师大学报（自然科学版）》
CAS
CSCD
2001年第3期88-92,共5页
Journal of Nanjing Normal University(Natural Science Edition)
基金
南京师范大学引进人才基金 (2 0 0 1SWXXGQB911)
青年科技基金 (2 0 0 0SWX0 0 0 0XQ1)资助
