摘要
目的 :在 15 3例肝病患者中分析新型肝炎病毒TTV的感染状况。方法 :通过设计特异引物采用半巢式PCR反应进行血清标本的TTVDNA检测。结果 :在 15 3例肝病患者中 ,第 1轮PCR未见明显的扩增带 ,在第 2轮PCR中 3 3例具扩增带 ,阳性率为 2 1 6%。其中 3 1例慢性肝炎中 6例呈阳性 ,2 4例肝硬化中 2例阳性 ,6例肝癌和 4例急性肝炎中各 1例呈阳性 ,88例不明原因的肝功能异常者中 2 3例呈阳性。而 8例肝功能正常者无 1例呈阳性。结论 :通过检测在肝病患得中存在TTV感染 。
Objective:To investigate the incidence of transfusion transmitied virus(TTV)infection in patients with liver diseases.Mothods:DNAs of TTV were amplified by semi-nested polymerase chain reaction(PCR)with specific primers.Results:TTV DNA wasn't detected in 8 normal persons.For first-sound PCR.the clear amplified band wesn't found in 153 patients with liver diseases,but for second-round PCR,the bands were displayed in 33 cases.The positive rate was 21.6% in patients.There were 6,2,1,1 and 23 cases in patients with chronic HBV/HCV hepatitis,cirrhosis,liver cancar,acute hepatitis and hepatitis of unknown etiology respectively.Conclusion:The results of the study suggest that TTV infections are existing in liver disease patients TTV may be a new type of hepatits,virus.
出处
《武警医学院学报》
CAS
2001年第4期297-299,共3页
Acta Academiae Medicinae CPAPF
