摘要
目的研究大黄素抗肝纤维化作用。方法 采用４０％四氯化碳给大鼠皮下注射制备肝纤维化模型并以小、中和大剂量大黄素（２０ｍｇ／ｋｇ、 ４０ｍｇ／ｋｇ、和 ８０ｍｇ／ｋｇ体重）干预，测定肝功能、血清透明质酸、层粘连蛋白及肝组织胶原蛋白，并通过光镜观察肝组织病理变化，免疫组织化学法检测肝组织α－肌蛋白表达。结果大黄素组较模型组：（１）肝功能明显改善：谷氨酸转氨酶及碱性磷酸酶显著降低，总蛋白及白蛋白显著升高；（２）血清透明质酸及层粘连蛋白显著降低；（３）肝组织胶原蛋白含量明显减少；（４）肝组织纤维化程度明显改善；（５）肝组织α－肌动蛋白表达减少。结论 大黄素具有抗肝纤维化作用。
Objective To investigate the effect of emodin on hepatic fibrosis in rats and study its possible mechanism. Methods The rat hepatic fibrotic model was induced by the subcutaneous injection of 40% CCl4 (twice a week for 6 weeks). The fibrotic rats were treated with low-dose, mediate-dose and high-dose emodin (20,40 and 80 mg/kg body weight, once a day for 42 days). Liver function, serum hyaluronic acid, laminin, and liver hydroxyproline were determined, Histopathological changes were examined by optical microscopy. The expression of a -smooth muscle actin (α -SMA) in liver tissue were detected by immunohistochemical techniques. Results Compared with model group, it revealed that in emodin-treated rats: (1) Liver functions was improved, alanine transaminase (ALT) and alkaline phosphatase (AKP) obviously reduced (P<0.05 or <0.01), total protein (TP) and albumin (ALB) significantly increased (P<0.05 or< 0.01). (2) Serum hyaluronic acid and laminin markedly reduced (P<0.05 or< 0.01). (3) Liver hydroxyproline were significantly decreased (P <0.05 or <0.01) (4) The degrees of fibrosis were reduced (P<0.05). (5) The expression of a-SMA in liver tissue were ameliorated. Conclusions Emodin has an effect on hepatic fibrosis in rats. The effect may be related to slowing hepatocyte injury and inhibiting liver a-SMA expressions.
出处
《中华肝脏病杂志》
CAS
CSCD
2001年第4期235-236,239,共3页
Chinese Journal of Hepatology
关键词
肝纤维化
大黄素
肝功能
动物模型
Hepatic fibrosis
Enodin
Liver function
Animal model
