摘要
分离自不同地区、不同动物的 30株厌氧真菌菌株分两次进行发酵降解试验。发酵底物为麦秸 ,其干物质含量为 5g·L-1,利用气压转换技术测定菌株发酵的产气量。试验Ⅰ 10个菌株降解麦秸所得的表观干物质消失率为 46 6～ 5 79mg·g-1,总产气量为 110～ 15 0mL。试验Ⅱ 2 0个菌株的表观干物质消失率为 386～ 5 94mg·g-1,总产气量为 99～ 144mL。两次试验中共有 2 4个菌株的总产气量与表观干物质消失率成正相关 ,相关系数 (R2 )分别为 0 917和 0 90 3。除菌株W 6外 ,2 9个菌株在 1/ 2气体总量时的生长速率相似 ,为 0 0 32～ 0 0 42h-1。菌株W 6的生长速率为 0 0 2 5h-1。 30个菌株的延滞期为 15 4～ 30 2h。结果说明 ,利用气压转化技术测定发酵产气量能方便有效地用于厌氧真菌生长动力学的比较和菌株的筛选 。
Anaerobic fungal isolates from different animals in a number of countries were investigated for in vitro fermentation in two batches with 10 and 20 isolates,respectively. Wheat straw at a concentration of 5 g·L -1 of dry matter was fermented and gas production profiles were determined using pressure transducer technique.The dry matter losses for the 10 isolates in batch one ranged from 466 to 579 mg·g -1 and the total gas production ranged from 110 to l50 mL.For the isolates in batch two,their dry matter losses were in a range of 386 to 594 mg·g -1 and the total gas production in a range of 99 to 144 mL.The total gas production for 24 isolates was directly proportional to their corresponding dry matter loss with R 2 as 0 917 and 0 903 for the two experiments,respectively.The growth rates for 29 isolates were similar,with the values ranging from 0 032 to 0 042 h -1 at the time for their half total gas production.The growth rate for isolate W6 was 0 025 h -1 .The lag times for all isolates ranged from 15 4 to 30 2 h.The results suggest that gas measurement determined using the pressure transducer technique is well suited to comparative studies of growth kinetics and screening of gut anaerobic fungi,without the need of destructive sampling.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2001年第3期44-48,共5页
Journal of Nanjing Agricultural University
基金
国家自然科学基金 (39870 5 78)
国家杰出青年科学基金 (30 0 2 5 0 34)资助
