摘要
The binary vectors which respectively contain chimeric genes (SCaM1-GFP, SCaM4-GFP) were constructed and used to transform tobacco (Nicotiana tabacumL.), pGTV-GFP was used as control. The plasmolyzed cells of transgenic callus treated with CBW were investigated under the laser scanning confocal microscope. Green fluorescence was found in the cell wall of transgenic SCaM1-GFP callus. However there was no green fluorescence in the cell wall of SCaM4-GFP or GFP transgenic callus. These results indicate that SCaM1 can be secreted into the apoplast of plant cells, while SCaM4 does not exist in the apoplast of plant cells.
The binary vectors which respectively contain chimeric genes (SCaM1-GFP, SCaM4-GFP) were constructed and used to transform tobacco (Nicotiana tabacum L.), pGTV-GFP was used as control. The plasmolyzed cells of transgenic callus treated with CBW were investigated under the laser scanning confocal microscope. Green fluorescence was found in the cell wall of transgenic SCaM1-GFP callus. However there was no green fluorescence in the cell wall of SCaM4-GFP or GFP transgenic callus. These results indicate that SCaM1 can be secreted into the apoplast of plant cells, while SCaM4 does not exist in the apoplast of plant cells.
基金
国家自然科学基金重点项目 ( 39730 2 30 )
国家重点基础研究规划项目 (G19990 1170 0 )
教育部科学技术研究重点项目 ( 0 0 14 0 )
国家杰出青年科学基金 ( 30 0 2 5 0 2 4)
教育部骨干教师基金~~
