摘要
从斜纹夜蛾核多角体病毒 (Spodopteralituranucleopolyhedrovirus,SpltMNPV)基因组中克隆了 gp37基因 .分子生物学软件分析表明 ,在 gp37基因内部存在糖基化位点 .含有晚期表达基因的启动子序列(ATAAG) ,推测为晚期表达的病毒膜糖蛋白基因 .通过GP37蛋白的同源性比较 ,绘制了以 gp37基因为基础的杆状病毒进化树 ,发现与以多角体蛋白基因 (polyhedrin)为基础所绘制的杆状病毒分子进化树有较大差异 .以polyhedrin基因为基础绘制的杆状病毒分子进化树将家蚕核多角体病毒 (Bombyxmorinucleopolyhedrovirus,BmNPV)与杆状病毒代表种———苜蓿丫纹夜蛾核多角体病毒 (Autographacalifornicamultinucleocapsidnucleopolyhedrovirus,AcMNPV)分开 ,根据 gp37基因分析则将二者归到同一分枝 。
The gp37 genes of Spodoptera litura NPV (SpltMNPV) was cloned and sequenced. Employing computer analysis, glycosilated sites and the promoter motif existed in the gp37 gene. and was shown as a late gene encoding a 37 ku glycoprotein. A phytogeny tree was designed by the comparison of their homologues from known gp37 genes of baculoviruses. It was shown that the phylogeny of baculoviruses on the basis of gp37 gene was different from that based on polyhedrin genes. For example, Bombyx mori NPV (BmNPV) and Autographa californica NPV (AcMNPV) were evolved in the same branch, otherwise, they were put in two distinct branches in the tree according to the analysis of polyhedrin. This result was consistent with the baculovirus phylogeny based on EGT published previously.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2001年第5期677-682,共6页
Progress In Biochemistry and Biophysics
基金
研究获国家自然科学基金重点项目 (3 973 0 0 3 0 )
"九五"国家重点科技攻关项目 (96 0 1 0 4)资助~~
关键词
斜纹夜蛾核多角体病毒
gp37基因簇
克隆
序列分析
Spodoptera litura nucleopolyhedrovirus (SpltMNPV)
gp37 gene cluster
cloned and sequenced
