摘要
目的 探讨6 0 Co辐照后免疫功能改变及其与白色念珠菌肠道定植的关系。方法 预先口饲白色念珠菌悬液 ,3d后行6 0 Co一次全身照射 (剂量分别为 0、4.5及 6.0Gy)后第 9天采用MTT法测定小鼠腹腔中性粒细胞杀菌功能及脾T淋巴细胞的增殖活性的同时 ,定量研究小鼠肠道白色念珠菌的定植。结果 辐射后第 9天两辐照组 (4 .5、6.0Gy)的腹腔中性粒细胞杀菌率、脾T淋巴细胞增殖活性显著低于对照组 (0Gy) (P <0 .0 1 )。而白色念珠菌的肠道定植量 (对数值 )显著高于对照组 (P <0 .0 1 )。相关分析显示 ,中性粒细胞杀菌率及脾T淋巴细胞增殖活性均与白色念珠菌的肠道定植量呈负相关 (P <0 .0 1 )。结论 6 0 Coγ射线一次全身辐照削弱了小鼠脾T淋巴细胞增殖活性 ,降低了腹腔中性粒细胞杀菌力 ,促进了白色念珠菌的肠道定植 。
Objective To explore the relationship between immunocompromise and the colonization of C.albicans in intestine of mice after 60 Co irradiation.Methods Thirty mice were randomly divided into three groups namely control (0 Gy), 4.5 Gy and 6 Gy 60 Co irradiation C.albicans suspeusion was given to the mice orally for 3 days. Poulation level of C.albicans in the cecal content, capacity of polymorphonuclear leucocytes (PMNL)killing C.albicans and T lymphocyte proliferation were determined quantialtively by MTT methods on the 9th day after radiation. Results Capacity of PMNL killing C.albicans of the two irradiation groups decreased to 37.68%(4.5 Gy), 26.84%(6 Gy) respectively and T lymphocyte proliferation decreased to 1.518,1.320 respectively, both were significantly lower than that of control (0 Gy)( P <0.01), while colonized C.albicans increased by 1 2 order of magnitude. Correlation analysis showed that negative relationship exist between PMNL killing capacity/T lymphocyte proliferation and colonized population of C.albicans in intestine of mice. Conclusion The decrease of capacity of PMNL killing C.albicans and T lymphocyte proliferation after irradiation increase the durability of intestine to C.albicans and promote the colonization of C.albicansin intestine of mice.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第10期1136-1138,共3页
Journal of Third Military Medical University
