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酿造工业中黑曲霉孢子检测方法的改良

Checking method of aspergillus niger spore improved in brewage industry
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摘要 根据黑曲酶孢子菌的特征,对其检测技术进行探讨。筛选出检测无菌液,找出适于此类菌系检测方法关键技术。研究结果表明,以0.15%琼脂无菌液代替无菌水对样品进行稀释,每个稀释液制成后,用磁力搅拌器搅拌,可使样品达到均匀分散程度,不再产生上浮、聚集的不均匀现象,再经接种、培养,即可获得准确结果,从根本上解决了用原稀释平板法检测孢子菌类数值重现性差、难以确定真值的问题。 Basing upon Aspergillus niger spore character, we dis- cussed checking technique screened out aseptic liquid and found out suitable key techonology of checking Asperbilus niger. Re- search result has indicated that aseptic liquid and 0. 15 percent of agar can be used to replace aseptic water to dilute sample. Every diluting liquid crank out. It is beat up by beater of magnetic force and obtains equal decentralization product. It does not float and congregate. Later it is inoculated and cultured. Thus the exact result can be gained. It can radically resolve the prob- lems that numerical repeat is low diluting flat method to check spore and in using no real numeral can be canfirmed.
作者 梁峙
出处 《广西轻工业》 2001年第3期46-47,30,共3页 Guangxi Journal of Light Industry
关键词 酿造 黑曲霉孢子菌 无菌液 稀释平板法 检测方法 改进 aspergillus niger spore asepsis liquid diluting flat method
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参考文献2

  • 1[1]赵继鼎、徐连旺、张小青.现代食品发酵技术.北京科学出版社,1981.
  • 2[2]王叔淳、林忠平、林志彬.食品卫生检验技术手册.化学工业出版社,1985.

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