摘要
目的 :观察心脉神口服液对动脉粥样硬化 (AS)的预防作用。方法 :将 18例日本大耳白兔随机分为3组 :正常对照组 (NCP) ,高胆固醇对照组 (HCP)和心脉神口服液预防组 (XP)。采用氧化酶法 ,紫外分光光度法测定血中总胆固醇 (TC)、甘油三酯 (TG)和丙二醛 (MDA)的含量 ;用病理观察、缺口末端标记法 (TUNEL)和L AB免疫组织化学法检测平滑肌细胞 (SMC)凋亡和增殖的表达变化。结果 :心脉神口服液降低血脂 (P<0 .0 5 )、降低丙二醛 (MDA,P<0 .0 5 )的作用明显 ;主动脉平滑肌增殖核抗原 (PCNA)表达减少 (P<0 .0 5 ) ,SMC凋亡增加 (P<0 .0 5 )。结论 :心脉神口服液除能降血脂、抗氧化、抑制 PCNA表达来减轻 AS外 ,也可以对 SMC凋亡有诱导作用从而逆转 AS的形成。
Objective:To observe the effects of Xinmaishen oral liquid(XMSol,心脉神口服液) on inhibiting effect on atherosclerosis(AS).Methods:Eighteen rabbits were selected and randomly divided into three groups:normal control group of prevention(NCP, n= 5);high cholesterol diet control group of prevention(HCP, n= 6);XMSol group of prevention(XP, n= 7).The levels of total cholestrol(TC),triglyceride(TG),and malondialdehyde(MDA) in serum were examined.The expression of proliferating cell nuclear antigen(PCNA) and apoptosis of aortic smooth muscle(SMC) in AS of dietinduced rabbits were observed by insitu terminal deoxynucleotidyl transferase mediated dUTP nick endlabeling(TUNEL) and LAB immunohistochemistry.Results:Prevention of XMSol has the effects of decreasing the levels of serum TC,TG,MDA(all P <0 05),reducing the expression of PCNA obviously( P <0 05),the expression of TUNEL could be found to be increased in group XP( P <0 05).Conclusions:XMSol shows remarkable preventive effects on experimental AS by decreasing bloodlipid,antioxidation,inhibiting the hiperplasia of SMC,and possesses the effect of increasing the expression of SMC TUNEL.
出处
《中国中西医结合急救杂志》
CAS
2001年第6期355-359,共5页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
北京军区"九.五云梯计划"基金资助项目( No.95 B0 5 2 )
