摘要
目的观察银杏叶提取物(GBE)对动脉粥样硬化模型大鼠血管内皮细胞的影响,并初步阐明其机制。方法将15只SD大鼠随机分为对照组、模型组、GBE组。用高胆固醇饲料喂养12周制备动脉粥样硬化大鼠模型。GBE组给予GBE水溶液96mg·kg-1·d-1灌胃,12周后取主动脉进行组织病理学检查,以电泳迁移率变动分析法(EMSA)检测核转录因子-κB(NF-κB)活性,用放射免疫法测定血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平,用酶联免疫吸附试验测量可溶性血栓调节蛋白(sTM)水平。结果电镜下显示,模型组血管内皮细胞存在明显的崩解现象,而GBE组损伤轻微。与对照组比较,模型组主动脉NF-κB活性、血清TNF-α、IL-6和sTM水平均显著升高,GBE组也有升高,但明显低于模型组(P均<0.05)。NF-κB活性与TNF-α、IL-6、sTM水平呈显著直线相关,sTM与TNF-α、IL-6呈显著直线相关(P均<0.05)。结论GBE可以通过抑制主动脉中NF-κB的活性、减轻过度的炎症反应而发挥保护内皮细胞的作用。
Objective To examine the effect of Ginkgo biloba （银杏叶） extract （GBE） on the endothelial cells in rat models with atheroselerosis and its mechanism. Methods Fifteen Sprague Dawley （SD） rats were randomly divided into three groups： high lipids （model） group, GBE group and normal control group. The rats in the model group were fed with high lipids and were sacrificed 12 weeks later. Besides fed with high lipids, the animals in GBE group were also administrated intragastriely with GBE aqueous solution （96mg·kg^-1·d^-1）. The aortas were harvested for histomorphometry and transmission electron microscope observation after 12 weeks. The nuclear factor-κB （NF-κB） activity was measured by electrophoretic mobility shift assays （EMSA） in aortas. The levels of tumor necrosis factor-alpha （TNF-α） and interleukin-6 （IL-6） in serum were measured by radio-immunity method and the level of soluble thrombomodulin （sTM） in serum was measured by enzyme linked immunosorbent assay （ELISA）. Results Under electron microscope, the necrosis and apoptosis of aorta endothelial cells were obvious in high lipids group, but the cell damage in GBE group was mild. The level of sTM in the GBE group was significantly higher than that in the normal control group and lower than that in the high lipids group （both P〈0.05）. The activity of NF-κB, the levels of IL-6 and TNF-α were evidently higher in the high lipids group than those in the normal control group, and in the GBE group, they were also elevated, but obviously lower than those in the model group （all P〈0. 05）. There were positive linear relationships between the NF-κB activity and each of the following levels： TNF-α, IL-6 and sTM Call P〈0.05）. The level of sTM also had linear relationship with the levels of TNF-α and IL-6 respectively （both P〈0.05）. Conclusion These results indicate that GBE can protect endothelial cells by inhibiting the activity of NF-κB and inflammatory response.
出处
《中国中西医结合急救杂志》
CAS
2008年第3期152-154,F0003,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
广东省科技计划基金资助项目(2004B30601016,2006B36004010)
广东省自然科学基金资助项目(5001676)
