摘要
目的 为判断紫杉醇能否有效抑制腺样囊性癌 (ACC)细胞增殖提供实验依据。 方法 通过紫杉醇作用于体外培养的 ACC- 2细胞及 L 92 9细胞 ,采用 MTT法测定细胞活性 ,计算并比较紫杉醇、顺铂 (CDDP)、5 -氟脲嘧啶 (5 - Fu)和阿霉素 (ADM)的 Cmax浓度时的细胞增殖抑制率、IC50 和相对抗肿瘤活性 (RAA)。 结果 Cmax浓度的紫杉醇对 ACC- 2的增殖抑制率为 (70 .46± 1.0 6 ) % ;紫杉醇、CDDP、5 - Fu和 ADM的 IC50 分别为 0 .0 2 ,2 .93,47.0 8和 0 .6 7((g/ m l) ,RAA分别为 18.0 ,1.0 ,0 .2和 0 .6。 结论 四种药物均有抗 ACC- 2细胞增殖作用及抗癌特异性。紫杉醇的单药活性最高 ,抗癌特异性与 CDDP和
Objective\ To investigate whether paclitaxel inhibited ACC\|2 cells efficiently or not, and provide valuable experiment data for clinical treatment of ACC.\ Methods\ In vitro, MTT assay was performed to detect proliferation inhibition rate of paclitaxel\|treated, CDDP\|treated, 5\|Fu\|treated or ADM\|treated ACC\|2 cells.\ IC\-\{50\} and RAA were used to evaluate single agent activity of these agents.\ L929 was used as control group to compare the anti\|cancer specificity of these four agents.\ Results\ With the same C\-\{max\} concentration the inhibition rate of proliferation on paclitaxel, CDDP, 5\|Fu, ADM, ACC\|2 were 70 46%±1 06%, 69 74%±1 83%, 39 44%±4 24% and 52 24%±2 33% respectively; IC\-\{50\} were 0 06, 2 93, 47 08 and 0 67 respectively; RAA were 18 0, 1 0, 0 2 and 0 6 respectively.\ But inhibition rate on L929 cell line were 30 02%±1 10%, 24 56%±2 13%, 56 67%±2 89% and 35 21%±1 61% respectively.\ Conclusion\ Paclitaxel inhibited ACC\|2 cell proliferation in vitro effectively.\ It had the highest single agent activity among these four anti\|tumor drugs.\ It had good anti\|tumor specificity as well as CDDP, 5\|Fu and ADM.\;
出处
《福建医科大学学报》
2001年第3期241-243,共3页
Journal of Fujian Medical University
