摘要
本文报道用几种碘标记方法(Iodosen、ChT、NBS、Bolton-Hunter试剂等)制备了^(125)I(^(131)I)-抗CEA单克隆抗体。产品通过Sephacex G-50(或-75)柱凝胶过滤纯化后,经纸层析和高压液相层析法鉴定,其放化纯度达到使用要求;用免疫放射双抗体夹心法测定,标记抗体能保持良好的免疫活性。同时我们对上述几种碘标方法进行比较研究,结果NBS法标记率最高,达98％,Iodogen法免疫活性保持最好,与CEA的净结合数达9960cpm(3ng CEA/250μl)。
The preparation of 125I(131I)-anti-CEA monoclonal antibody by using several radio-iodination methods (Iodogen, ChT, NBS, and Bolton-Hunter reagent etc.) was described. Purification of the product was carried out by the Sephadex G-50 (or 75) gel-filtration column. Its radiochemical purity checked by paper chromatography and HPLC was satisfactory for use. The labelled antibody showed good immunoreactivity, when it was measured by IRMA-bi-antibody sandwich method. The comparative studies for above several radioiodination methods were carried out, among which the iodination efficiency of NBS method was the best (98%) while the immunoreactivity of lodogen method was the best and specific binding of labelled antibody to CEA was 9960 cpm (3ng CEA/ 250 μl).
出处
《核技术》
CAS
CSCD
北大核心
1989年第11期648-652,共5页
Nuclear Techniques
关键词
单克隆抗体
碘标记法
IODOGEN法
Iodogen method ChT method NBS method Bolton-Hunter reagent method IRMA HPLC
