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钙调素的间接碘标记 被引量:1

INDIRECT IODIZATION LABELLING OF CAMODULIN
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摘要 以间接碘标记蛋白质的方法制备125I-CaM,比活度为1.56MBq/μg,125I的总标记率为23.1%。实际应用表明,由于减少了直接碘化标记对抗原的损伤,其特异性、稳定性等技术指标均能满足放射免疫工作的要求。 he iodinated calmodulin with 125I by the indirect iodination method is prepared.The specific radioactivity of 125ICaM is 1.56 MBq/μg,the total labelling rate of 125I is 23.1%. Clinical tests indicate that the specificity,stability and other technical specifications of the iodinated CaM can satisfy the needs of RIA for reducing the harm of direct iodination to the antigen.
出处 《核化学与放射化学》 CAS CSCD 北大核心 1998年第2期119-122,共4页 Journal of Nuclear and Radiochemistry
关键词 钙调素 间接标记 碘标记 标记 碘125 碘化标记 Calmodulin 125I Indirect labelling
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  • 1张丽民,核技术,1987年,9卷,6期,46页

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  • 1李晓军,武建国,孙大业,邵海枫.重组人钙调素对白芷细胞增殖及结核杆菌生长的影响[J].医学研究生学报,2000,13(4):234-237. 被引量:1
  • 2[1]Flier JS. What's In a name ? in search of Leptin's physiogic role[J]. J Clin Endocr Meta,1998,83(5):1407-1413.
  • 3[5]Bolton AE, Hunter WE. The labeling of proteins to high specific radioactivity by conjugation to a 125I-containing acylating agent[J]. Biochem J,1973,133(15):529-536.
  • 4[6]Montague CT, Prins JB, Sanders L et al. Depot-and sex-specific differences in human leptin mRNA expression. Implications for the control of regional fat distribution[J]. Diabets, 1997,46(3):342-347.
  • 5[7]Sainsbury A, Cusin I, Doyle P et al. Intra-cerebroventricular administration of neuropeptide Y to normal rats increases obese gene expression in white adipose tissue[J]. Diabetologia, 1996,39(2):353-360.
  • 6[8]Sih R, Morley JE, Kaiser FE et al. Testosterone replacement in older hypogonadal men: a 12-month randomized controlled trial[J]. J Clin Endocrinol Metab, 1997,82(6):1661-1667.
  • 7郗晶涛.肥胖基因产物——Leptin的研究进展[J].国外医学(内分泌学分册),1998,18(3):129-132. 被引量:13

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