摘要
目的 观察锌对血管细胞凋亡的影响。方法 选用雄性新西兰大白兔 2 1只 (实验组 14只 ,对照组7只 ) ,实验组饮水中加葡萄糖酸锌 (锌浓度 15 .0 mmol/L )喂养 ,分别于 6,12个月时取主动脉 ,应用光镜及电镜观察血管形态学变化 ,以 DNA末端标记法观察细胞凋亡。结果 锌喂养 6个月时微细结构及超微结构出现不同程度的病理变化 ,12个月时血管内壁色泽灰暗 ,弹性降低 ,内皮细胞肿胀 ,中膜平滑肌增生 ,细胞核膜内陷切迹 ,染色质回缩 ,线粒体部分空泡变 ,胞浆膜破裂 ,弹力纤维稀疏。实验组较对照组血管细胞凋亡增多 (P <0 .0 1) ,细胞凋亡面积增加 (P <0 .0 1)。结论 补锌能诱导血管细胞凋亡 。
Objective To observe influence of zinc on apoptosis in vascular.Methods Twenty one New Zealand white rabbits were chosen.They were divided into 2 groups (control group and experimental group).The rabbits of experimental group were fed by zinc glucose in drinking water.The changes of aorta were observed after the rabbits were fed for 6 months and 12 months.Results Fine structure and ultrastructure of aorta appeared pathological change after 6 months and 12 months.The chnges showed that wall of vessel became murky grey,its plasticity decreased,that endothelial cells swelled and smooth cells proliferated,both cells appeared vacuola.Ultrastructure changes were nuclear membrane broken,plasticity fibril loose.The numbers of apoptotic cells and apoptotic area were more increased in vessels of experimental group than that of control group (P<0.01).Conclusions Supplemental zinc may induce apoptosis of vascular cells.The changes may be one of important mechanism which arteriosclerosis was caused by zinc.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2002年第1期25-26,共2页
Chinese Jouranl of Endemiology
基金
山东省卫生厅科研基金资助项目 ( CA1DK A 1)
