摘要
目的研究转移因子口服液中附加剂对转移因子鉴别和含量测定的影响。方法鉴别采用紫外分光光度法 ;含量测定采用福林 酚法。结果山梨酸钾与转移因子紫外吸收图谱相似 ,严重干扰转移因子鉴别试验 ;甜蜜素、葡萄糖酸锌、薄荷脑对鉴别无影响。山梨酸钾、甜蜜素、葡萄糖酸锌、薄荷脑对福林 酚法测定转移因子口服液中多肽含量均无显著干扰。结论转移因子口服液不宜用紫外作鉴别试验 ;处方中含有山梨酸钾、甜蜜素、葡萄糖酸锌、薄荷脑时 ,仍可选择福林
PurposeThe aim is to investagate the effects of additives in trasfer factor on ultraviolet spectrum identity and polypeptide content assay.MethodsUltraviolet spectrum were applied for identity and Folin phenol method for polypeptide content assay of trasfer factor respectively.ResultsPotassium sorbite interferes seriously ultraviolet spectrum for identity of trasfer factor . Potassium sorbite,sweetening agent,zinc gluconate,menthol do not interfere polypeptide content assay of trasfer factor.ConclusionWhen the solution of transfer factor contains potassium sorbite, using ultraviolet spectrum for identity is not suitable.
出处
《中国生化药物杂志》
CAS
CSCD
2001年第5期244-245,共2页
Chinese Journal of Biochemical Pharmaceutics
