摘要
目的 优选全氟显与新型超声仪最佳条件组合 ,评价心肌声学造影 (MCE)的应用价值。方法 开胸犬模型分三组 :条件优选组按 4(剂量 )× 3(机械指数 )× 2 (探头频率 )析因试验设计行MCE并定量分析比较 ;缺血再灌注(IR)组前降支动脉缺血 1h ,再灌注 2h ;缺血预适应组 (IP)于缺血 1h前先予缺血 5min和再灌注 5min ,反复 4次。取出心脏以Evans蓝和TTC双重染色测定危险心肌与梗死心肌 ,并同MCE法作相关分析和组间比较。结果 三种参数均影响MCE效果 ,但无交互作用 ;MCE测的梗死和危险心肌范围与病理双染指标呈密切相关 ;MCE同病理观察一致呈示IP显著缩小梗死面积。结论 MCE需合适的条件组合 ;MCE可同时准确测定梗死与缺血心肌范围 ;
Objective To optimize the optimal parameters of fluorocarbon contained agent combined with intermittent harmonic ultrasound and to evaluate the application of myocardial contrast echocardiography (MCE). Methods Anesthetized open chest dogs were divided into three groups: Optimal condition group devised according to the scheme 4(doses)×3(mechanic index)×2(transducer frequencies) of factorial analysis were assessed by MCE quantitatively; Ischemia reperfusion group (IR) subjected to 1 hour of left descending coronary artery occlusion followed by 2 hours of reperfusion while ischemic preconditioning group (IP) were given to 5min ischemia and 5min reperfusion for 4 times before 1 hour of occlusion and 2 hours of reperfusion. The correlation of MCE and pathologic staining with Evan's blue and triphenyltetrazolium chloride in identifying myocardial area at risk and infarct size at 2 hours after reperfusion were analyzed. The use of MCE in investigating IP was also evaluated versus pathological method. Results Three factors all affects the MCE image, but they haven’t interaction; Indexes of necrotic and risk area by MCE had an excellent correlation with that by pathologic stain; With accordance to pathological method, MCE showed IP decreased myocardial infarct size significantly. Conclusion MCE needs suitable combination of the parameters;MCE can accurately defined myocardial area at risk and infarct size; MCE can use in IP investigation.
出处
《中国医学影像技术》
CSCD
2001年第7期615-617,共3页
Chinese Journal of Medical Imaging Technology
基金
国家自然科学基金资助部分课题 (C39870 32 9)
关键词
心肌声学造影
间歇二次谐波
全氟显
缺血预适应
Myocardial contrast echocardiography
Intermittent harmonic
Fluorocarbon
Ischemic preconditioning
