摘要
目的 评价间歇二次谐波心肌声学造影（ ｍｙｏｃａｒｄｉａｌ ｃｏｎｔｒａｓｔ ｅｃｈｏｃａｒｄｉｏｇｒａｐｈｙ ，ＭＣＥ） 在梗死心肌再灌注研究中的价值。方法 用前降支冠状动脉套扎建立开胸犬模型。分两组：缺血再灌注组于血流阻断１ ｈ 后再灌注２ ｈ ；缺血预适应组则在缺血１ ｈ 前预缺血和再灌注各５ ｍｉｎ ，反复４ 次。两组均于再灌注２ ｈ 后经静脉注射全氟显进行ＭＣＥ，随后取出心脏用Ｅｖａｎ 蓝和ＴＴＣ 染色，对照两种方法所测的心肌坏死面积、缺血危险面积及坏死占缺血危险面积的百分比。结果 ＭＣＥ 所代表的坏死、缺血危险面积及坏死百分比与组织学染色所代表的相应的实际参数一致（ 分别为ｒ ＝ ０ ．９５ 、ｒ ＝ ０ ．９４ 和ｒ ＝ ０ ．９７ ， Ｐ ＜ ０ ．０１） 。结论 ＭＣＥ 可替代组织染色法作为评估梗死心肌再通后心肌坏死与缺血的有效方法。
Objective Myocardial contrast echocardiography (MCE) allows the possibility to detect myocardial perfusion in vivo.To assessed its accuracy in identifying myocardial area at risk and infarct size by intermittent harmonic MCE.Methods Anesthetized open chest dogs were classified into two groups:Ischemia and reperfusion group (IR) subjected to 1 hour of left descending coronary artery (LDA) occlusion followed by 2 h of reperfusion while ischemic preconditioning group (IP) was given to 5 min ischemia and 5 min reperfusion for 4 times before 1 h of occlusion and 2 h of reperfusion.Intermittent harmonic MCE using intravenous injection of contrast agent C 3F 8 was assessed at 2 h after reperfusion in identifying myocardial area at risk and infarct size versus gross pathologic specimens stained with Evan′s bule and triphenyltetrazolium chloride (TTC).Results The area at risk and infarct size and percents of necrotic area determined by intermittent harmonic MCE had a excellent correlation with that by pathologic stain (r= 0.95 , 0.94 and 0.97 ,respectively,P< 0.01 ).Conclusions Intermittent harmonic MCE using intravenous C 3F 8 accurately defined myocardial area at risk and infarct size,which may provide a method in vivo for animal experiment and clinical investigation of myocardial ischemia and reperfusion.
出处
《中华超声影像学杂志》
CSCD
2000年第1期58-60,共3页
Chinese Journal of Ultrasonography
基金
国家自然基金!(C39870329)
关键词
间歇二次谐波
心肌再灌注
超声心动图
Echocardiography
Intermittent harmonic
Myocardial reperfusion
