摘要
目的：建立高效毛细管电泳快速分离和测定丹参水溶性有效成分的方法。方法：以pH为7.0，含0.5 mmol·L-1十六烷基三甲基溴化铵, 浓度为180 mmol·L -1 的磷酸盐缓冲液与乙腈按40∶11 (v/v)混合配制电泳缓冲液，在75 μm(id)× 34.5(eff.30) cm的毛细管柱上，10 kV(6 s)电迁移进样，运行电压为8 kV，紫外检测 波长为200 nm, 柱温控制于20 ℃。结果：在8 min内能将以乙腈-水(20∶80)为溶剂的样品溶液，即内标(对羟基苯甲酸)、丹参素、 原儿茶醛和原儿茶酸完全基线分离，柱效达40万/m理论塔板数，以丹参素、原儿茶醛和原儿 茶酸与内标的峰面积比值为定量指标，定量检测限为0.1 μg·ml-1，线性范围 为5.0～0.1 μg·ml-1，方法学的日内和日间变异均小于6%。结论：本法快速、高效、简便，结果准确可靠。
OBJECTIVE To establish an HPCE method for determining the water-soluble active constituen ts of the Radix. METHODS Capillary electrophoresis conditions:capillary,75 μm(id)(34.5(eff.30) cm, uncoated;column temperature,20 ℃;detector,UV 200 nm;injection,10 kV/6 s;running voltage,8 kV;buffer,180 mmol·L-1 phosphate buffer(pH 7.0,containing 0.5 mmol·L-1CTAB) -acetonitrile (40∶11,v/v).Sample solvent was ac etonitrile-water (2∶8,v/v). RESULTS Good separation was achieved in 8 min.The column effeciency reduced 400000 tho retical plate number per metre.The limit of quantification was 0.1 μg·ml -1.The good linear range was from 5.0 μg·ml-1 to 0.1 μg·m l-1.The within-day and between-day relative standard deviations of stan dard solutions were less than 6%. CONCLUSIONS The method was found to be accurate and convenient.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2001年第10期582-584,共3页
Chinese Journal of Hospital Pharmacy
