摘要
目的 通过体外细菌生物被膜模型观察司帕沙星对生物被膜内细菌的清除作用。方法 以平板培养法建立体外粘液型铜绿假单胞菌生物被膜模型。双倍稀释法测定司帕沙星 (SPL X)和头孢他啶 (cef-tazidime,CAZ)的最低杀菌浓度 (MBC)和对浮游细菌的杀菌曲线。将附有生物被膜的硅胶片用盐水冲洗后与1～ 12 8倍 MBC的两种抗生素共同孵育 48h。将硅胶片在超声作用下使细菌剥脱于培养基中 ,细菌计数后换算成每平方厘米硅胶片上细菌数的常用对数 (log cfu/ cm2 ) ,最后绘出对生物被膜内细菌的杀菌曲线。结果 对浮游细菌的杀菌作用 CAZ优于 SPL X。对生物被膜内细菌的杀菌活性 ,两种抗生素在 2 MBC浓度时均不能清除细菌 ,SPL X在 32 MBC浓度下 2 4h即完全清除细菌 ,而 CAZ在 12 8MBC浓度下 48h仍有少量细菌未能清除。讨论 由于司帕沙星对生物被膜具有较好的通透性 ,且其杀菌作用不依赖细菌的生长率 ,故对生物被膜内细菌具有良好的清除作用 。
Objective: To explore the eradication effect of sparfloxacin SPLX on biofilm bacteria with biofilm models in vitro . Methods: The MBCs of sparfloxacin and ceftazidime were measured and planktonic bactericidal curves were draw. The plate culture method was used to establish in vitro P.aeruginosa biofilm model. After the models were completely established, the silicon pieces with biofilm were cultured with two antibiotics at 1~128 MBC concentrations respectively for 48 hours. The silicon pieces were treated with ultrasound to separate the biofilm bacteria, and the viable bacteria were counted. At last, the biofilm bactericidal curves were drawn. Results: The activity of ceftazidime on free bacteria was stronger than that of sparfloxacin. Conversely, sparfloxacin has more potent biofilm bactericidal activity than ceftazidime. The biofilm bacteria were completely eradicated by sparfloxacin in 24 hours at a concentration of 32 times MBC. But, the biofilm bacteria still remained by ceftazidime at a concentration of 128 times MBC for 48 hours. Conclusion: Results suggested that sparfloxacin has stronger eradication on biofilm bacteria.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2001年第5期390-392,共3页
Chinese Journal of Antibiotics
