摘要
目的了解华勒变性过程中雪旺细胞的形态学变化。方法选用42只SD大鼠,在腋部切断正中神经和尺神经,以远侧段作为预变神经。术后当天、3d和1、2、3、4、8周(1～7组)取材,通过雪旺细胞培养、S-100蛋白标记及电子显微镜技术观察雪旺细胞数量和髓鞘厚度。结果神经被切断后,雪旺细胞的数量开始增加,预变后1周达到高峰,约为犤(3.52±0.27)×107犦个/ml。神经被切断后,髓鞘的厚度也相应增加,预变后1周达到最厚,约为(231.2±8.9)nm,随后缓慢变薄。结论在华勒变性过程中,雪旺细胞的数量和髓鞘厚度随变性时间的增加而发生变化。
Objective To study the morphological changes of the Schwann cells within the predegenerated nerve. Methods The ulanr and the median nerves were cut bilaterally at the axillary level in 42 SD rats. A nerve segment of 1 cm in length was harvested from the distal segment of each of the cut nerves right after the operation, 3 days, 1, 2, 3, 4 and 8 weeks postoperatively(group 1-7). The methods of Schwann cells culture and S-100 protein standing were used to study the temporal changes of the number of the Schwann cells, and the thickness of myelin of the predegenerated nerve were studied under electromicroscope. Results The number of Schwann cells increased after nerve transection, and reached a peak of [(3.52±0.27)×107]/ml in one week. The thickness of myelin also increased after nerve transection, and was thickest at about (231.2±8.9) nm in one week. The myelin thickness slowly decreased after this time. Conclusion The number of the Schwann cells and the thickness of myelin of the predegenerated nerve were changed with time.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
2001年第11期689-691,共3页
Chinese Journal of Orthopaedics
基金
1999年国家教育部优秀青年教师基金资助项目
2000年上海市教育基金会"曙光计划"资助项目
