摘要
目的应用双酶消化法来提取高纯度和高数量的周围神经雪旺细胞。方法对双酶消化周围神经的时间由３０分钟延长至８０～９０分钟；机械分离周围神经束的方法改为在放大１６倍手术显微镜下分离神经束。对培养细胞进行雪旺细胞数量、Ｌｏｗｒｙ蛋白含量的测定；并用同位素标记测定细胞量和免疫组织化学法观察。结果从成年ＳＤ大鼠１ｃｍ长一段上臂尺神经中，用该法可提取１．７２×１０７个雪旺细胞／毫升，雪旺细胞的纯度和数量是以往所有提取方法中最高的，并且这些雪旺细胞的细胞形态和再生功能均正常。结论该改良方法为研究周围神经再生的机理，提供了一个重要的手段。
Objective To introduce a new technique of controlled enzyme digestion of peripheral
nerves to obtain a highly purified suspension of Schwann cells. Methods The peripheral
nerve specimen was digested by two enzymes for 80 to 90 minutes. The nerve fascicle were
then extracted under 16 microscope instead of naked eyes. The number of Schwann cells was
counted after isotopic labeling.The content of L protein was examined by S 100 protein
staining. Results An average of 1.72 107 cells/ml was obtained from 1 cm segment of the ulnar
nerve in the grown up SD rat. The cells were highly purified, with normal morphology and
function. Conclusions This modified method for extraction and culture of Schwann cells
provides a new means for the study of the mechanism of peripheral nerve regeneration.
出处
《中华手外科杂志》
CSCD
1999年第2期106-108,共3页
Chinese Journal of Hand Surgery
基金
美国中华医学基金
上海市领先学科基金
关键词
神经再生
许旺氏细菌
细胞培养
免疫酶技术
Nerve regenerationSchwann cellCulture,CellImmunoenzyme
techniquesRats,SpraqueDawley
