摘要
目的 探讨大鼠急性胰腺炎 (AP)时胰腺缺血和细胞因子过度释放的关系。方法 以牛磺胆酸钠诱导大鼠AP ,2 0只为急性水肿性胰腺炎 (AEP)模型、2 0只为急性坏死性胰腺炎 (ANP)模型 ,另取 10只正常大鼠作为对照。术后 12h处死各组 10只大鼠 ,取血和胰腺组织 ,检测肿瘤坏死因子α(TNF α)和白细胞介素 10 (IL 10 )水平 ,观察胰腺病理变化 ,并以多普勒超声仪检测大鼠胰腺血流(PBF)。结果 AEP大鼠血清和胰腺中TNF α和IL 10水平均升高 ,分别为 ( 186± 13 )、( 2 10± 3 0 )pg/ml和 ( 660± 2 9)、( 669± 62 )pg/ml;ANP大鼠两者分别为 ( 3 3 7± 5 6)、( 4 43± 60 )pg/ml和 ( 12 4± 12 )、( 2 0 2± 3 8)pg/ml。AP时PBF明显减少 ,PBF波幅与血清和胰腺组织中TNF α和IL 10水平及病理评分密切相关 (P值均 <0 0 1)。结论 AP大鼠PBF的减少与细胞因子的增高同时发生 ,两者都是AP的重要致病因素 ,又互为因果 ,相互促进。
Objectives To investigate the r el ationship between pancreatic ischemia and cytokines released in rats with acute pancreatitis(AP). Methods 20 acute edematous pancreatitis(AEP) and 20 acute necrotizing pancreatits(ANP) rat models wer e induced by injection of sodium taurocholate into the pancreatic duct, another 10 normal rats were used as control. At 12 hours after the induction of AP, 10 r ats in each group were sacrificed, blood and pacreatic tissue samples were taken for measurement of TNF-α and IL-10 levels. The pathological study of pancre as was performed, and pancreatic blood flow(PBF) was measured by Doppler ultraso und instrument.Results The TNF -α and IL-10 levels in serum and pancreatic tissue increased after the induction of AP, IL-10 levels elevated more significantly in AEP rats, TNF-α levels e levated more significantly in ANP rats. PBF reduced in AP rats, and the amplitud e of PBF measured by Doppler ultrasound was closely correlated with serum and ti ssue TNF-α, IL-10 levels, inflammation, hemorrhage and necrosis scores.Conclusion The reduction of PBF and the i ncreasement of cytokines developed simultaneously in rats with AP, hence both of them are important pathogenic factors of AP.
出处
《中华普通外科杂志》
CSCD
北大核心
2001年第11期662-664,共3页
Chinese Journal of General Surgery
