摘要
选用不同来源的转 Bt基因抗虫棉中棉所 30号和新棉 33B、转 tfd A基因抗除草剂棉花材料 TFD,通过与不同主栽推广品种正反交 ,研究了外源 Bt基因和 tfd A基因在棉株体内的遗传和分离行为 .结果表明 ,由于外源基因提供给转基因棉花的抗虫和抗除草剂性状均是由一对于基因控制的显性性状 ,且该性状不受细胞质效应的影响 。
Three transgenic cotton varieties(lines)were chosen for the study of inheritance of foreign Bt ( Bacillusthuringiensis toxin )and tfdA genes in cotton The transformed cotton varieties CCRI 30 and NewCott 33B expressing the BtcryIA gene,and cotton 1ine TFD expressing the tfdA gene were crossed with CCRI 19,CCRI 12 and Lumain 6 The results confirm inheritance and segregation of (i) the exogenous Bt gene in transgenic CCRI 30 and NewCott 33 B, governing resistance to bollworm,and (ii) the exogenous tfdA gene in transgenic TFD,governing resistance to the herbicide 2,4 D Both resistance characters were governed by a single dominant nuclear gene, and were not affected by cytoplasm Our data support the conclusion that foreign traits encoded by single genes are inherited and expressed in Mendelian fashion in cotton Our results also indicate that a practical backcross breeding program could be used to develop cotton cultivars combining one or more resistance traits from foreign and native gene sources
出处
《生命科学研究》
CAS
CSCD
2001年第4期345-350,共6页
Life Science Research
基金
河南省自然科学基金项目 ( 9840 110 0 0 )~~
