摘要
为了建立简单有效地筛选抗卡那霉素标记基因玉米的方法,分别用玉米自交系"昌7-2"和杂交种"郑单958"为试验材料,用不同浓度、不同体积的卡那霉素溶液分别浸种3、4 d后播种,调查播种10 d后的玉米幼苗白化率,确定玉米对卡那霉素的抗性,结果表明用卡那霉素浸泡玉米种子,玉米苗的白化率不但与卡那霉素浓度有关,而且与浸种的用液量有关,杂交种和自交系之间差异并不大,利用100 mL浓度为200 mg/L的卡那霉素溶液浸种3 d后播种,白化苗率基本可以达到100%,用100 mL浓度为150mg/L的卡那霉素溶液浸种20粒种子3 d后播种进行抗性筛选,经过对玉米T1代转基因植株的筛选试验进行验证准确度达到了84.8%,表明该方法可行,可作为筛选玉米转化抗卡那霉素标记基因的一种简便方法。
In order to establish simple and effective method of screening marker gene in maize which was resistant to the kanamycin,the corn inbred line ＇Chang 7-2＇ and hybrid ＇Zhengdan 958＇ was used as the test material.First,the corn seeds were soaked with different concentrations of kanamycin solution for 3 or 4 d,respectively,then sown,and the bleaching rate of maize seedlings for 10 days after sowing was investigated,finally the corn resistance to kanamycin was determined.The results showed that the bleaching rate of corn seedlings was not only related with the concentration of kanamycin,but also with the amount of soaking liquid,and almost had no differences between hybrids and inbred lines.Seeds which were soaked with 100 mL of the concentration of 200 mg/L kanamycin for 3 d were sown,the rate of albino seedlings was basically up to 100 %.In order to improve the accuracy of screening,it was suggested that 20 seeds were soaked with 100 mL of the concentration 150 mg/L kanamycin for 3 d and then carryed out to test screening for resistance.After verifying the screening test of T1 transgenic maize plants,the accuracy was up to 84.8 %.It demonstrated the feasibility of the method,which could be used as a simple method of screening transfer gene maize of kanamycin resistance.
出处
《西南农业学报》
CSCD
北大核心
2010年第6期1895-1899,共5页
Southwest China Journal of Agricultural Sciences
基金
转基因生物新品种培育重大专项(2009ZX08010-011B)
