摘要
DNA分子标记技术是检测遗传差异的一种新技术 ,特别是RAPD技术 ,应用更为广泛。DNA质量是保证RAPD分析成功的关键。本文用改进的SDS法对沙拐枣属植物种子的总DNA进行了提取。琼脂糖凝胶电泳结果表明 :得到的DNA片段大小在 2 0kb以上 ;通过PCR扩增实验 ,证明用此方法提取的DNA ,可直接用于随机扩增的DNA多态性 (RAPD)
DNA Molecular Markers is a new method for dete cting genetic variation. RAPD(Random amplified polymorphic DNA) technique has been widely used in genetic and systematic evolution of plant. The quality of DNA sample is important in RAPD analysis . In this paper, an improved SDS method of DNA isolation was applied to obtain total DNA from \%Calligonum\% spp. The results of agarose gel electrophoresis showed that the fragments of DNA samples were larger than 20 kb; The PCR results showed that high level of genetic variation was found between four \%Calligonum \%species. It was also proved that the isolated DNA could be used directly for RAPD analysis which are useful for detecting the genetic diversity of \%Calligonum\%.
出处
《中国沙漠》
CSCD
北大核心
2001年第3期300-302,共3页
Journal of Desert Research
基金
中国科学院沙坡头开放试验站基金资助
