摘要
目的 标化HCV RNA定量国家标准品,研制HCV RNA基因诊断试剂第二代国家参考品。方法 从各地收集肝炎病人和供血员血样,用HCV RNA PCR试剂检测样品,用ROCHE试剂确证,用HCV基因分型试剂确定HCV基因型别。以WHO国际标准品标化国家HCV RNA定量标准品。邀请有关单位会同标定。结果 建立了HCV RNA定量国家标准品,研制了HCV RNA第二代国家参考品。结论 发现国产HCV RNA PCR试剂灵敏度和特异性需进一步改进。
Objective To calibrate the national HCV RNA standard according to WHO ' s standard material and prepare the second national reference panel for HCV RNA reagents.Methods Sera of blood donors and hepatitis patients were collected and detected for HCV RNA, confinned with ROCHE HCV RNA PCR kits. The HCV genotypes were detected using INNO - LiPA HCV Ⅱ. Results The first HCV RNA national standard were set up and the second national reference panel for HCV RNA reagents were prepared. Conclution The sensitivity and specificity of domestic HCV RNA PCR kits should also be improved.
出处
《中国预防医学杂志》
CAS
2001年第3期164-167,共4页
Chinese Preventive Medicine
基金
国家"九五"重点科技攻关基金资助(96-906-03-06)
