摘要
目的 分析抗 -HCV阳性献血者血清 HCV RNA含量与 ALT活性的关系。方法 采用 ELISA法检测抗 -HCV,阳性标本用荧光 PCR试剂盒测定 HCV RNA,分析标本 ALT活性与 HCV RNA水平的关系。结果 60份抗 -HCV阳性标本经 PCR检测后有 2 7份标本含有 HCV RNA,HCV RNA拷贝量与 ALT活性呈正相关 ,且标本的 HCV RNA含量越多 ,ALT活性也越高 ( P<0 .0 1 )。结论 献血者中筛查 ALT并结合 PCR技术 ,能减少 HCV经血传播的危险。
Objective To investigate the relationship betweem serum hepatitis C virus RNA and ALT levels in anti-HCV positive samples of donors. Methods Hepatitis C virus fluorescence PCR diagnostic kit was carried out on the anti-HCV positive samples in order to reveal any possible relationship between HCV RNA and ALT levels. Results 27 samples were tested positive for HCV RNA among 60 anti-HCV positive samples. The more copies of HCV RNA in the blood samples, the higher the ALT levels(P<0.01). Conclusion The combination of ALT testing with HCV RNA PCR in donor screening can reduce the risk of transmission of HCV through blood transfusion.
出处
《临床输血与检验》
CAS
2004年第3期171-172,共2页
Journal of Clinical Transfusion and Laboratory Medicine
