摘要
根据毛里塔尼亚果蝇Drosophilamauritania的mariner转座子序列设计简并引物 ,以白背飞虱Sogatellafurcifera和灰飞虱Laodelphaxstriatellus基因组DNA为模板 ,通过PCR反应扩增出了约 5 0 0bp的条带 ,经回收、克隆、测序 ,获得其核苷酸序列 ,与毛里塔尼亚果蝇mariner序列相比 ,核苷酸同源性均为 71 3%,氨基酸同源性分别为 6 9 5 %和 72 %,证明白背飞虱和灰飞虱体内确实存在mariner转座子。
About 500 bp DNA fragment is amplified by PCR with the degenerated primers designed according to the mariner sequence of Drosophila mauritiana, the genomic DNA of either Sogatella furcifera or Laodelphax striatellus as the template. Sequence analysis shows that the fragment similarity of both the DNA to that of D. mauritiana is 71 3%, and the similarity of the amino acid is 69 5% and 72% respectively. This indicates that the mariner element may exist in S. furcifera and L. striatellus.
出处
《昆虫学报》
CAS
CSCD
北大核心
2001年第4期402-407,共6页
Acta Entomologica Sinica
基金
国家自然科学基金资助项目 (3990 0 0 93)
