摘要
根据产气荚膜梭菌产生 4种主要毒素 ( α,β,ε,ι毒素 )可分为 A～ E5种菌型 .依据产气荚膜梭菌的 5种毒素基因的序列 ,设计 5对 PCR引物 ,建立多重聚合酶链式反应 ( PCR)方法 ,以快速区分 5种类型的产气荚膜梭菌 .6 8份环境样品 (生活污水、屠宰场污水 )的常规检测和基因分型共检出 5 5株产气荚膜梭菌 ,其中 A型产气荚膜梭菌 5 1株 ,占总数的 90 %以上 ,B,C,D 3种类型只占 5 % ,试验未检出 E型产气荚膜梭菌 ,所有的分离株未发现带有肠毒素 .结果表明 :目前污水中主要存在的菌型为 A型菌 ,其他菌型的产气荚膜梭菌很少 。
Clostridium pertringens isolates are currenty classified into one of five biotype (AE) on the basis of the differential production of α,β,ε ,and ι toxin. Different biotypes are associated with different diseases of man and animals. In this study a multiplex PCR assay was developed to detect the genes encoding five major toxins ( α,β,ε,ι toxin and enterotoxin). C. perfringens isolates taken from sewage samples, including sewages and slaughter water were genotyped using multiplex PCR assay. The results showed that most isolates were found to be genotype A, over 90% of total C. Perfrigens isolates, only about 5% of C. Perfringens isolates were proved to be other types, entero toxigenic C. Pertringens were not found in this study.
出处
《扬州大学学报（自然科学版）》
CAS
CSCD
2001年第4期27-30,共4页
Journal of Yangzhou University：Natural Science Edition
