摘要
目的 探讨丹参对内毒素休克性肺损伤的防治作用。方法 选用SD大鼠 5 4只 ,随机分成对照组、肺损伤组和丹参防治组。三组动物根据注入内毒素后时间不同分为 1、2和 4小时三小组。丹参防治组按 8g/kg体重经颈静脉注入丹参 ,30分钟后 ,再按 5mg/kg体重经颈静脉注入大肠杆菌内毒素 ;肺损伤组以生理盐水代替丹参 ;对照组中 ,丹参和内毒素均以等量生理盐水代替。实验结束后 ,取血和肺组织行血浆P 选择素、肺组织MPO活性、肺毛细血管通透性和血液流变学检测。结果 (1)肺损伤组各时相点血浆P 选择素、肺组织MPO活性、肺毛细血管通透性均较对照组显著增高 (P <0 0 1) ;丹参防治组较肺损伤组有所下降 ,以 4小时为甚 (P <0 0 1)。 (2 )肺损伤组血液流变学指标较对照组明显升高 (P <0 0 1) ;丹参防治组与肺损伤组比较 ,血液流变学指标有不同程度的降低 ,以高中切变率 ηb和ERI差异有显著 (P <0 0 5或P <0 0 1)。 结论 丹参通过改善血液流变性 ,抑制P
Objective To investigate the preventive effects of salvia militiorrhiza on endotoxic shock induced lung injury.Methods 54 SD rats were divided into three groups:control group,lung injury group and salvia miltiorrhiza pretreatment group(SM).Each group was subdivided into three small groups according to the time of endotoxin adminstrated at 1h,2h and 4h.Six animals were included in each small group.After thirty min of salvia miltrorrhiza in a dose of 8mg/kg was given.Escherichia coli O 111 B 4 lipopolysaccharide in a dose of 5mg/kg was intravenously administrated in SM group.In endotoxin group,salvia miltiorrhiza was used instead of equivalent normal saline,where as in control group,alvia miltiorrhiza and endotoxin were used instead of equivalent normal salin.After the experiment,arterial blood and lung was obstained for measurements of plasma P selectin,lung MPO,pulmonary capillary permeability and homrrheological.Results (1)Plasma P selectin levels,lung MPO,pulmonary capillary permeability at every time points in endotoxin group was much greater than those observed in control group( P< 0 01).Those in SM group were decreased compared with those in endotoxin group,especially at 4h point( P< 0 01).(2)Homrrheological parameters,in endotoxin group,including plasms viscosity,whole blood viscosity,index of red cell aggregation(EAI) and index of red cell rigidity(ERI) were noticeably increased compared with those in control group.These indexes in SM group decreased to some extent compared with those in endotoxin group,but only whole blood viscosity at high or middle sheer rate and ERI had obvious difference.Conclusions Salvia miltiorrhiza may modify blood rheology and inhibit PMN infiltration through P selectin,which provides protective effects on lung injury. [
出处
《临床麻醉学杂志》
CAS
CSCD
2001年第11期616-618,共3页
Journal of Clinical Anesthesiology
