摘要
目的 研究人脑胶质瘤组织中尿激酶型纤溶酶原激活剂 (uPA)及其受体 (uPAR)的表达 ,探讨uPA、uPAR的表达与人脑胶质瘤恶性程度的关系。方法 采用半定量逆转录—聚合酶链反应 (RT PCR)方法 ,对 49例人脑胶质瘤手术切除标本、U2 5 1等 3株胶质瘤细胞 ,12例内减压术中切除的正常脑组织标本的uPAmRNA和uPARmRNA表达水平进行检测。结果 随着胶质瘤恶性度的升高 ,其uPA及uPARmRNA表达率和表达水平逐渐增高。U2 5 1等 3株胶质瘤细胞也表达了较高水平的uPA及uPARmRNA ,而正常组织表达率及表达水平极低。结论 人脑胶质瘤中纤溶酶原激活系统活性较高 ,uPA、uPAR基因的高表达反映了胶质瘤的恶性生物学行为。
Objective To study the expression of urokinase type plasminogen activator and its receptor in gliomas, and analysis the relationship between uPA/uPAR expression and the grade of human gliomas. Methods In surgical sample of 49 patients with glioma, 3 glioma cell lines and 12 samples of normal brain tissues obtained from craniocerebral injury surgery, the level of uPA/uPAR mRNA were studied using semi quantitative reverse transcription polymerase chain reaction. Results Along with the rise of glioma grade, the uPA mRNA and uPAR mRNA expression rate and level were also upregulated. U251 and other 2 glioma cells had a high level of uPA and uPAR mRNA, but the rate and level of uPA and uPAR mRNA of normal brain tissues were extremely lower. Conclusions The plasminogen system activation was quite higher in gliomas. The high level of uPA and uPAR mRNA reflecte a malignant biological behavior of glioma cells.
出处
《中华神经外科杂志》
CSCD
北大核心
2001年第5期283-286,共4页
Chinese Journal of Neurosurgery
基金
江苏省非教学系统留学回国人员科技活动D类基金资助 ( 1999年度 )
