摘要
目的 :建立毛细管电泳分离分析人类精子碱性核蛋白的新方法。方法 :Beckman聚丙烯酰胺涂层柱 ,总长 47cm ,有效长度 40cm。缓冲液 :2 0mmol·L-1柠檬酸 ,pH用 0 0 1mol·L-1氢氧化钠溶液调至 3 0 ;分离电压 :14kV ;柱温 :18℃ ;分离时间 2 0min ;检测波长 :2 14nm ;每次运行之间用缓冲液洗柱 4min ;压力进样 6～ 9s。结果 :所建立HPCE分析方法能够将HP1、HP2及HP3有效分离 ,迁移时间及峰高重现性分别小于 2 0 %及 10 % ,能够对大鼠及人精子碱性核蛋白进行快速分析。结论 :所建立方法比传统的凝胶电泳方便。
Objective:To establish a new capillary electrophoresis method for the analysis of human sperm protamine.Method:Polyacrylamide coated column was used.The total length was 47 cm and the effective length was 40 cm.Running buffer consisted of 20 mmol·L -1 citric acid at pH adjusted to 3 0 by 0 1 mol·L -1 NaOH.Electrophoresis was performed at 15 kV and 20 ℃.The detection wavelength was 214 nm.The capillary was rinsed with running buffer for 5 min between each run.The sample was introduced into the capillary by pressure injection for 6～9 s.Results:HP1,HP2 and HP3 in human sperm protamine were effectively separated by the established method.The RSDs of migration time and peak height were less than 2 0% and 10% respectively.Both rat and human sperm protamines could be analysed rapidly.Conclusion:The established method is easier and faster than the conventional gel electrophoresis method.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2001年第5期323-325,共3页
Chinese Journal of Pharmaceutical Analysis
