摘要
目的 :建立适合国人尿液中头孢克洛分离分析的毛细管电泳方法。方法 :用重蒸馏水多倍稀释尿液后直接进样电泳分析。电泳分离条件为 :未涂层毛细管 48cm× 5 0μm ;运行缓冲液为 5 0 m mol/ L磷酸二氢钠 - 12 .5 mm ol/ L硼砂 (p H 8.0 8) ;操作电压 2 0 k V(+ )→ (- ) ;压力进样 8.2 74× 10 4Pa· s;柱上紫外 2 6 5 nm检测。 结果 :头孢克洛和内标物头孢拉定在 6 .5min内获得了理想的分离。尿药浓度在 2 .5～ 2 5 0 .0μg/ ml范围内线性关系良好 (r =0 .9991,n =5 ) ,绝对回收率大于95 .5 % ,日内及日间精密度均小于 8.0 %。 结论 :毛细管电泳应用于健康志愿者口服头孢克洛普通胶囊后尿药浓度的动态分析 ,方法准确可靠 。
Objective:To establish capillary electrophoretic(CE) method for the separation and determination of cefaclor in human urine.Methods:Urine samples were injected directly into the capillary after multi fold dilution.The electrophoretic separation was achieved with 50 mmol/L phosphate 12.5 mmol/L borate (pH 8.08 ) running buffer and a constant voltage of 20 kV was applied to the 48 cm×50 μm uncoated capillary(Supelco, USA). The analytes were introduced into capillary by pressure(8.274×10 4 Pa·s) and determined with on column monitoring at 265 nm.Results:An ideal separation for cefaclor and internal standard cefradine in human urine was completed within 6.5 min by CE. The analytical results of cefaclor urine samples demonstrated a good linear relationship within the range of 2.5 250.0 μg/ml. The absolute recoveries were more than 95.5% and the relative standard deviations of inter day and intra day were less than 8.0%.Conclusion:The CE method is accurate, simple, rapid and has been applied successfully in dynamic analysis of urine samples after a single oral dose of 500 mg cefaclor capsule in 6 male healthy volunteers.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第5期469-472,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金资助项目!(39700181)
关键词
毛细管电泳
头孢克洛
尿药浓度
electrophoresis,capillary
cefaclor
urinary drug level
