摘要
为建立抗 -HCV的时间分辨免疫荧光分析法 (IFMA) ,以HCV抗原包被微孔板 ,样品中的抗 -HCV、Eu3 + 标记羊抗人IgG形成夹心 ,以β -二酮体为增强液 ,数据采用Log -Logit函数和四参数Logistc函数数据处理程序处理。结果表明 ,方法的批内和批间CV分别为 3.6 4 %和 4 .39% ,平均回收率 10 5 .5 8% ,可测范围为 1.0 1— 17.34NCU/mL ,灵敏度为 0 .0 3NCU/mL。本法与HB sAg有 0 .2 3%的交叉 ,与HBeAg有 0 .0 4 %的交叉。 2 78例正常对照组血清抗 -HCV浓度均小于1NCU/mL。结果提示 ,高度灵敏、稳定的抗
To establish an IFMA of anti-HCV, the antigen of HCV was covered on the microwell plate, and then incubated with human anti-HCV in sample and Eu 3+ labeled goat anti-human IgG. The luminescent enhancement solution contains mainly 2-napthoyltrifluororacetate, and the data was treated with the log-logit function and four- parameter logistic function. Results showed that the intra- and inter- assay CV of the IFMA was 3.64% and 4.39%, respectively, and the recovery rate was 105.58%, the sensitivity was 0.03NCU/mL. The cross reactivity with HBsAg and HbeAg was 0.23% and 0.04%, respectively. The anti-HCV concentrations in serum of 278 health persons were all less than 1NCU/mL. Results indicated this new method was sensitive, stable and could be used as an effective non-radioassay tool for the basic or clinical study on HCV.
出处
《核技术》
CAS
CSCD
北大核心
2001年第11期891-894,共4页
Nuclear Techniques
