摘要
以 CA1 2 5单克隆抗体包被微孔板 ,用平衡饱和法与 CA1 2 5、Eu3+标记异硫氰酸苄基二乙烯三胺四乙酸 -CA1 2 5单抗形成三层夹心 ,以β-二酮体为增强液 ,建立 CA1 2 5的时间分辩免疫荧光分析法 ( IFMA)。方法的批内和批间 CV分别为 2 .4 5%、2 .66% ,平均回收率 1 0 4 .2 9% ,灵敏度 3.2 7U/L,可测范围为 6.0 5-2 0 62 U/L。本法与 CEA无交叉 ,与 AFP有 6.77%的交叉 ,与β-HCG有 1 0 .89%的交叉。 84名健康女性血清 CA1 2 5浓度为 1 1 .4 2± 1 2 .2 0 U/L,34例卵巢癌患者血清 CA1 2 5浓度为 334 .33± 376.66U/L,与对照组比较有高度显著性差异 ( P<0 .0 1 )。血清样品测定结果与酶免疫分析 ( ELISA)和化学发光免疫分析 ( CLIA)测定结果呈相关 ,其相关系数 ( r)分别为 0 .888和 0 .899。诊断试验结果表明 ,CA1 2 5IFMA的敏感度、特异性。
A two-site time resolved immunofluorometric assay (IFMA) of CA125 based on the direct sandwich technique has been developed on the equilibrium method. The monoclonal antibody (McAb) directed against CA125 was labelled with europium with the help of the europium-chelate of diethylenetriaminepentaacetic acid (DTPA). The luminescent enhancement system was an enhancement solution contained mainly 2-naphthoyltrifluoroacetone. The intra-and inter-assay CV of the IFMA were 2.45% and 2.66%, respectively, and the recovery rate was 104.29%, the sensitivity was 3.27U/L. The McAb provided a linear response from 6.05 to 2062U/L. The cross-reacting rate with CEA was negligible, and that with AFP and β-HCG was 6.77% and 10.89%,respectively. The serum concentration of CA125 by this method was 11.42 ±12.20U/L in 84 normal female volunteers, 334.33±376.66U/L in 34 patients who were suffered from ovariancus. The hight-relation of the results was found from IFMA, CLIA and ELISA( r : 0.888/ 0.899). CA125 IFMA was a good test for clinical application with brtter sensitivity, specificity, accuracy and predictive value.
出处
《标记免疫分析与临床》
CAS
2001年第2期96-99,共4页
Labeled Immunoassays and Clinical Medicine
关键词
CA125
单克隆抗体
时间分辨免疫荧光分析
Carbohydrate antigen(CA)
Time-resolved immunofluorometric assay Monoclonal antibody
