摘要
目的 探讨急性脑血管病患者血液中协同刺激分子白细胞分化抗原簇 80 (clusterofdifferention 80 ,CD80 ,B7 1)、白细胞分化抗原簇 86(clusterofdifferention 86,CD86,B7 2 )、白细胞分化抗原簇 2 8(clusterofdifferention 2 8,CD2 8)、细胞毒性T淋巴细胞抗原 4 (cytotoxicTlymphocyteantigen 4 ,CTLA 4 )蛋白的表达和B7 1、B7 2信使核糖核酸 (messengerribonucleicacid ,mRNA)的表达及其与急性脑血管病病损的关系。方法 应用分子生物学技术 ,检测急性期脑梗死 ( 2 6例 )、脑出血患者 ( 2 6例 )和年龄匹配的 2 6名健康对照者外周血淋巴细胞B7 1、B7 2、CD2 8和CTLA 4蛋白的表达和外周血淋巴细胞B7 1、B7 2mRNA的表达 ,各病例均经头部CT或MRI检查并计算病灶体积 ,采用改良爱丁堡 +斯堪的那维亚评分标准 (SSS)对临床神经功能缺损程度进行评分 ,分析其相互关系。结果 外周血淋巴细胞B7 1、CD2 8和CTLA 4蛋白的表达 ,脑梗死组高于脑出血组 ,两组均高于健康对照组 ;外周血淋巴细胞B7 2蛋白的表达 ,三组间无显著性差异 ;健康对照组外周血淋巴细胞未检测到CTLA 4蛋白的表达 ;外周血淋巴细胞B7 1mRNA的表达 ,脑梗死组高于脑出血组 ,两组均高于健康对照 ;外周血淋巴细胞B7 2mRNA的表达 ,三组间无显著性差异 ;?
Objective To explore the relationship between the expression of costimulating moleculars, cluster of differention 80(CD80,B7 1), cluster of differention 86(CD86,B7 2), cluster of differention 28(CD28), cytotoxic T lymphocyte antigen 4(CTLA 4), B7 1 messenger ribonucleic acid (mRNA), B7 2 mRNA, and extend of injury in acute cerebrovascular patients. Methods Using molbiology and gene technology,measured the levels of the expressed B7 1, B7 2, CD28, CTLA 4, and B7 1 mRNA, B7 2 mRNA in lymphocytes in periphery blood from 26 acute cerebral infarction, 26 cerebral hemorrhage and 26 age matched healthy control subjects,each case was determined by CT scans or MRI and was measured of the volume of the focus Analyzed the correlation between the expression of costimulating moleculars and the extent of the clinical neurological deficits which was accordingly estimated revised Scandinavianvian Stroke Scale(SSS). Results The level of expressed B7 1,CD28?CTLA 4 in lymphocytes in cerebral infarction were higher than those in cerebral hemorrhage patients Both of the two groups were elevated over the control group But there was no significant difference of B7 2 among the three groups No CTLA 4 was detected in normal controls The levels of B7 1mRNA in cerebral infarction were increased markedly over those in cerebral hemorrhage All of them were higher than normal group No group was siginifcantly different from others in the expression of B7 1mRNA There were positive correlations between the expression of B7 1, CD28 in cerebral infarction patients and SSS Both costimulating moleculars and the volume of the focus in cerebral hemorrhage were of no relationship with SSS But there were positive correlations between the volume of the focus in cerebral infarction patients and SSS. Conclusions Costimulating moleculars play important roles in the pathological mechanism of acute cerebrovascular disease Aiming at costinmulating moleculars,holding the activating of the inflammatory cells may provide a new way for the treatment of acute cerebralvascular disease,especially for cerebral infarction
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2001年第4期237-241,共5页
Chinese Journal of Neurology
基金
长沙市科学技术委员会科技专项计划项目 (长科计字 [1999] 0 7)
关键词
脑血管意外
CD80抗原
CD28抗原
分化抗原
信使RNA
Cerebrovascular accident
Antigens, CD80
Antigens, CD28
Antigens, differentiation
RNA, messenger
