摘要
目的 探讨槲皮素对糖尿病大鼠肾脏保护作用。方法 对链脲佐菌素诱导糖尿病大鼠动物模型 ,给予槲皮素 10 0mg·kg-1·d-1,治疗共 8周。放免法测定尿白蛋白排泄率 ,测定内生肌酐清除率及肾小球蛋白激酶C活力。RT PCR检测方法观察槲皮素对糖尿病大鼠肾脏皮质TGF β1基因表达的影响 ,观察槲皮素对肾小球形态及病理改变。结果 糖尿病大鼠存在肾小球高滤过 ,尿白蛋白排泄率、肾重 /体重、肾小球细胞膜蛋白激酶C活力明显升高。肾脏皮质TGF β1基因表达显著增加 ,肾小球肥大。给予槲皮素治疗 2周及 8周时 ,糖尿病大鼠肾小球滤过率、尿白蛋白排泄率、肾小球肥大均较糖尿病未治疗组显著降低 ,肾小球细胞膜蛋白激酶C活力及TGF β1基因表达显著下降。肾脏病理改变不显著。结论 槲皮素通过抑制糖尿病大鼠肾脏蛋白激酶C活力可以纠正糖尿病早期肾脏高滤过、高灌注 ,并同抑制肾脏皮质TGF β1基因过度表达有关 。
Objective To investigate the protective effect of Quercetin on kidneys in diabetic rats. Methods STZ induced diabetic rats were given quercetin 100 mg·kg -1 ·d -1 for 8 weeks. Urinary albumin excretion rate (UAER) was measured by radioimmunoassay. The changes of creatinine clearance rate (Ccr) and glomerular protein kinase C (PKC) activities were determined. The expression of TGF β 1 mRNA of renal cortex in diabetic rats were determined by RT PCR analysis. The glomerular changes were also observed morphologically. Results In untreated diabetic rats, Ccr, UAER, kidney weight/body weight and PKC activity in renal glomeruli were significantly increased, the expression of TGF β 1 mRNA in renal cortex was elevated, and glomerular hypertrophy existed. After Quercetin treatment, Ccr, UAER, PKC activity and the expression of TGF β 1 mRNA were markedly reduced as compared with those of untreated diabetic rats in 2 and 8 weeks, no significantly abnormal changes in kidney morphology were observed in Quercetin treated group. Conclusion Quercetin ameliorates early diabetic renal hyperdynamic abnormality via inhibiting PKC activity, in which inhibiting of TGF β 1 production seems to be involved. Reduction of the PKC activity is important in preventing or delaying the development of diabetic nephropathy.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2001年第5期316-319,共4页
Chinese Journal of Endocrinology and Metabolism
基金
全军"九五"医学科研基金资助项目 ( 98Q0 18)
