摘要
目的检测HCV基因及其抗原在人原发性肝内胆管细胞癌中的表达,探讨肝内胆管细胞癌的病因及发病机制。方法用免疫组化技术检测HCV抗原,用原位分子杂交技术检测HCV基因(HCV RNA)。结果在40例肝内胆管细胞癌中,HCVC33c抗原、核心抗原及HCV RNA阳性率分别为52.5％,72.5％和57.5％;在21例癌旁肝组织中,其阳性率分别为63.6％,45.5％和48.5％。结论人原发性肝内胆管细胞癌的发生除与HBV有密切关系外,与HCV慢性感染也有密切关系。
AIM In order to explore the etiology and pathogenesis of human primary intrahepatic cholangiocarcinoma, expression of HCV genes and its antigens in cancer and its surrounding hepatic tissues was detected. METHODS HCV antigens were detected by immunohistochemical technique and HCV genes by in situ hybridization. RESULTS Among 40 cases of intrahepatic cholengiocarcinoma, the positive rate of HCV C33c, core antigen and HCV RNA was 52.5%, 72.5% and 57.5%, respectively; in 21 cases of cancerous surrounding hepatic tissues, 63.6%, 45.5% and 48.5%, respectively. CONCLUSION In the pathogenesis of the intrahepatic cholangiocarcinoma, there exists a close relationship between human primary intrahepatic cholangiocarcinoma and besides HBV, and HCV chronic persistent infection.
出处
《世界华人消化杂志》
CAS
2001年第5期541-545,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金 No.39270744
关键词
原发性肝内胆管细胞癌
HCV基因
HCV抗原
发病机理
cholangiocarcinoma/pathology
bile ducts, intrahepatic/pathology
hepatitis C-like viruses
genes, virus
gene expression
hepatitis C antigens/biosynthesis
