摘要
应用原位分子杂交及免疫组化方法，使用地高辛标记ＨＣＶ５＇非编码区探针及抗ＨＣＶＮＳ３区Ｃ３３ｃ单克隆抗体检测３５例人原发性肝内胆管细胞癌，癌旁肝组织内ＨＣＶＲＮＡ及其ＮＳ３抗原，发现ＨＣＶＲＮＡ在肝内胆管细胞癌中的阳性率为８３％。ＨＣＶＲＮＡ定位于肝细胞胞浆中，个别病例在淋巴细胞、肝窦内皮细胞和枯否细胞中发现阳性信号。ＨＣＶＮＳ３区Ｃ３３ｃ抗原在肝内胆管细胞癌中的阳性率为８９％，阳性反应主要位于癌细胞胞浆内或肝细胞胞浆内，阳性细胞在癌组织中以灶性分布为主，在癌旁肝组织以弥漫分布为主。ＨＢ－ｘＡｇ在本组病例的阳性率为７７％。本文结果提示，在肝内胆管细胞癌的发生中，除ＨＢＶ以外，ＨＣＶ感染与其发生也有密切关系，是其致病因素之一。
HCV RNA and its NS3 region C33c antigen were detected by in situ hybridization and immunohis tochemical methods in 35 cases of primary intrahepatic cholangiocarcinoma(PIC ) tissues. The positive rate of HCV RNA in PIC was,83%and the HCV RNA was localized in the cytoplasm and/or nuclei of cancerous cell and hepatocytes. In one case,HCV RNA was also detected in nuclei of lymphocyte and sinusoidal cell/or Kupffer's cells. The positive rate of HBV NS3 region C33c antigen was 89%in PIC,positive reaction was found in the cyto- plasm of cancerous cell and hepatocytes,The positivc cells were distributed locally and diffusely. HBxAg was also detected in 77%of the patients studied.Our resuIts suggested that HCV infection close correlation with the de- velopment of PIC except HBV infection. HCV may play an important rloe in the carcinogenesis of PIC.
出处
《解放军医学杂志》
CSCD
北大核心
1996年第2期83-85,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金
关键词
胆管肿瘤
肝炎病毒
抗原
免疫组织化学
bile duct neoplasm
hepatitis B virus
antigen
virus
in situ hybridization
