摘要
目的 分析成年大鼠小脑基因与新生大鼠小脑基因的差异表达并获取新的表达序列标签。方法 成年大鼠小脑来源的cDNA为受检者 (tester) ,新生大鼠小脑的cDNA为驱动者 (driver) ,进行差减杂交 ,经克隆 ,获得成年大鼠小脑差减杂交库。结果 共挑选出 5 4个阳性克隆质粒 ,测序得到 5 6个不同基因片段序列 ,其中有 16个是大鼠中首次测得的表达序列标签 ,并被GenBank收录 (BG946 773～BG946 788)。
Objective To analyze differentially expressed gene in cerebellum of adult rat and to obtain a novel expressed sequence tag (EST).Methods The cDNA from cerebellum of adult rat was taken as 'tester', and those from cerebellum of 1 day postnatal rat as 'driver'. The suppression subtractive hybridization method was carried out . After cloning, a differential cDNA libraries was obtained.Results 54 clones were selected out and 56 ESTs were sequenced. 16 of 56 ESTs were identified to be novel ESTs and given the gene sequence numbers by GeneBank (BG946773~BG946788).Conclusion Unknown ESTs can be selected after differentially expressed gene libraries was constructed with the method of suppression subtractive hybridization.
出处
《同济大学学报（医学版）》
CAS
2001年第5期4-6,共3页
Journal of Tongji University(Medical Science)
基金
铁道部科技发展专项基金资助项目 (J99Z0 2 3)
关键词
消减杂交
表达序列标签
小脑
大鼠
基因克隆
suppression subtractive hybridization
expressed sequence tag
cerebellum
rat
