摘要
采用聚酰胺薄层层析分离、分光光度法 ,对一种海生超微型黄藻品系PP983的色素组成进行了研究测定。结果表明 ,该黄藻中只含有叶绿素a而不含叶绿素c,检出 8种类胡萝卜素 ,除作为黄藻特征色素的Heteroxanthin和Vaucheriaxanthin两种外 ,还发现该藻含有一个吸收峰在 471nm ,呈现多甲藻素 (peridinin)特征的色素。用高效液相色谱 (HPLC) ,并以微小多甲藻 (Peridiniumpusillum)作对照 ,对其光合色素作进一步的分析 ,确证该黄藻中不含有叶绿素c而含多甲藻素。结果显示此种黄藻与甲藻有更近的亲缘关系。
The pigment composition of a marine picoalgae strai n PP983 was analyzed to study the classification and evolution of Xanthophyta. T he results of polyamide thin-layer chromatogram and spectrophotography showed t hat this species contained eight types of pigments besides Chl-a, but no Ch l-c. Unexpectedly, in addition to the characteristic pigments of Xanthophyt a (heteroxanthin and vaucheriaxanthin), we also found a pigment whose maximal ab sorption was at 471 nm. This pigment displays the characteristic of peridinin. I n order to verify occurrence of peridinin in this species, extracted pigments fr om strain PP983 were separated and detected on HPLC with UV-Vis and fluorescenc e detectors by using Peridinium pusillum as a control. The results showed th at the Xanthophyta strain PP983 contains peridinin and suggested that Xanthophyt a have very close relation to Dinophyta. It provides a proof to the evolution an d phylogeny of Xanthophyta.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2001年第5期489-493,共5页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金资助项目 3 9770 5 90号
曾呈奎海洋科学基金 (1 999)资助项目。
关键词
黄藻
多甲藻素
Xanthophyta, Peridinin, Evolution
